Anti-Neurexin 1α (extracellular) Antibody | Alomone Labs ANR-031 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Neurexin 1α (extracellular) Antibody

catalog :

ANR-031

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Yotsumoto T, Maemura K, Watanabe K, Amano Y, Matsumoto Y, Zokumasu K, et al. NRXN1 as a novel potential target of antibody-drug conjugates for small cell lung cancer. Oncotarget. 2020;11:3590-3600 pubmed publisher
Twining R, Vantrease J, Love S, Padival M, Rosenkranz J. An intra-amygdala circuit specifically regulates social fear learning. Nat Neurosci. 2017;20:459-469 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates: - 1,2. Anti-Neurexin 1α (extracellular) Antibody (#ANR-031), (1:400).3,4. Anti-Neurexin 1α (extracellular) Antibody, preincubated with Neurexin 1α (extracellular) Blocking Peptide (#BLP-NR031).

image 2 :

Peptide (C)KPRHQKDAKHPQMIK corresponding to amino acid residues 546-560 of rat Neurexin 1? (AccessionQ63372). Extracellular N-terminus.

image 3 :

Peptide (C)KPRHQKDAKHPQMIK corresponding to amino acid residues 546-560 of rat Nrxn1? (AccessionQ63372). Extracellular N-terminus.

product information

CAT :

ANR-031

SKU :

ANR-031-CF_0.2 ml

Product Name :

Anti-Neurexin 1α (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63372

Applications :

IC IF LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR031

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KPRHQKDAKHPQMIK, corresponding to amino acid residues 546-560 of rat Nrxn1α

Product Page - Scientific background :

Neurexins (NRXNs) are a family of transmembrane, synaptic adhesion molecules. NRXNS were identified as receptors for α-latrotoxin, a presynaptic toxin that triggers massive neurotransmitter release1. Neurexins are largely presynaptic proteins that form a trans-synaptic cell-adhesion complex with postsynaptic neuroligins2. They are encoded by three genes (NRXN1, NRXN2 and NRXN3), each using an upstream promoter to produce the longer α-isoform (α-NRXNs) and a downstream promoter to generate a shorter β-isoform (β- NRXNs).The α- and β-isoforms of each neurexin are single-pass transmembrane proteins maintaining identical transmembrane and intracellular domains but having distinct extracellular domains. NRXNs in neurons localize to the presynaptic membrane and bind trans-synaptically to postsynaptic adhesion molecules and receptors3. Neurexins are expressed in all neurons, and are subject to extensive alternative splicing, generating >1,000 splice variants, some of which exhibit highly regulated developmental and spatial expression patterns4. The extracellular domain of neurexin 1α (NRXN1α) is composed of three neurexin repeats (I, II, and III), which each contains the modules LNS-EGF-LNS. NRXN1α binds endogenous ligands including neuroligins, LRRTM family members, neurexophilin, α-dystroglycan, and GABA(A) receptors.NRXN1α gene mutations have been identified in neuropsychiatric diseases including Schizophrenia and Autism Spectrum Disorder5.

Applications may also work in :

IC IF LCI WB

Supplier :

Alomone Labs

Target :

Nrxn1, Neurexin I-alpha

Short Description :

A Rabbit Polyclonal Antibody to Neurexin 1α

Long Description :

Anti-Neurexin 1α (extracellular) Antibody (#ANR-031) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and live cell imaging applications. The antibody recognizes an extracellular epitope and is therefore, highly suited to recognize neurexin 1α in living cells. It has been designed to recognize Nrxn1α from rat, mouse and human samples.

Negative Control :

BLP-NR031

Positive Control :

Synonyms :

Nrxn1, Neurexin I-alpha

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NRXN1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage