Anti-Neurexin 1α (extracellular)-ATTO Fluor-488 Antibody | Alomone Labs ANR-031-AG product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Neurexin 1α (extracellular)-ATTO Fluor-488 Antibody

catalog :

ANR-031-AG

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 488

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Yotsumoto T, Maemura K, Watanabe K, Amano Y, Matsumoto Y, Zokumasu K, et al. NRXN1 as a novel potential target of antibody-drug conjugates for small cell lung cancer. Oncotarget. 2020;11:3590-3600 pubmed publisher
Munder A, Israel L, Kahremany S, Ben Shabat Binyamini R, Zhang C, Kolitz Domb M, et al. Mimicking Neuroligin-2 Functions in β-Cells by Functionalized Nanoparticles as a Novel Approach for Antidiabetic Therapy. ACS Appl Mater Interfaces. 2017;9:1189-1206 pubmed publisher

image

image 1 :

Multiplex staining of Neurexin 1α and GABA(A) ε Receptor in mouse cerebellum - Immunohistochemical staining of rat cerebellum frozen section using Anti-Neurexin 1α (extracellular)-ATTO Fluor-488 Antibody (#ANR-031-AG) and Anti-GABA(A) ε Receptor (GABRE) (extracellular)-ATTO Fluor-633 Antibody (#AGA-015-FR). A. Nrxn1α staining (green). B. GABA(A) ε receptor staining (purple). C. Merge of the two images suggests extensive co-localization in Purkinje cells (arrow).

image 2 :

Immuno-colocalization of Neurexin 1? and GABA(A) ? Receptor in mouse cerebellum - Immunohistochemical staining of rat cerebellum frozen section usingAnti-Neurexin 1? (extracellular)-ATTO-488 Antibody(#ANR-031-AG) andAnti-GABA(A) ? Receptor (GABRE) (extracellular)-ATTO-633 Antibody(#AGA-015-FR). A. Neurexin 1? staining (green). B. GABA(A) ? receptor staining (purple). C. Merge of the two images suggests extensive co-localization in Purkinje cells (arrow).

product information

CAT :

ANR-031-AG

SKU :

ANR-031-AG_50 mcl

Product Name :

Anti-Neurexin 1α (extracellular)-ATTO Fluor-488 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63372

Applications :

IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR031

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KPRHQKDAKHPQMIK, corresponding to amino acid residues 546-560 of rat Nrxn1α

Product Page - Scientific background :

Neurexins (NRXNs) are a family of transmembrane, synaptic adhesion molecules. NRXNS were identified as receptors for α-latrotoxin, a presynaptic toxin that triggers massive neurotransmitter release1. Neurexins are largely presynaptic proteins that form a trans-synaptic cell-adhesion complex with postsynaptic neuroligins2. They are encoded by three genes (NRXN1, NRXN2 and NRXN3), each using an upstream promoter to produce the longer α-isoform (α-NRXNs) and a downstream promoter to generate a shorter β-isoform (β- NRXNs).The α- and β-isoforms of each neurexin are single-pass transmembrane proteins maintaining identical transmembrane and intracellular domains but having distinct extracellular domains. NRXNs in neurons localize to the presynaptic membrane and bind trans-synaptically to postsynaptic adhesion molecules and receptors3. Neurexins are expressed in all neurons, and are subject to extensive alternative splicing, generating >1,000 splice variants, some of which exhibit highly regulated developmental and spatial expression patterns4. The extracellular domain of neurexin 1α (NRXN1α) is composed of three neurexin repeats (I, II, and III), which each contains the modules LNS-EGF-LNS. NRXN1α binds endogenous ligands including neuroligins, LRRTM family members, neurexophilin, α-dystroglycan, and GABA(A) receptors.NRXN1α gene mutations have been identified in neuropsychiatric diseases including Schizophrenia and Autism Spectrum Disorder5.

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

Nrxn1, Neurexin I-alpha

Short Description :

A Rabbit Polyclonal Antibody to Neurexin 1α Conjugated to the Fluorescent Dye ATTO-488

Long Description :

Anti-Neurexin 1α (extracellular) Antibody (#ANR-031) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot and live cell imaging applications. It has been designed to recognize Nrxn1α from rat, mouse and human samples. Anti-Neurexin 1α (extracellular)-ATTO-488 Antibody (#ANR-031-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Neurexin 1α (extracellular)-ATTO-488 Antibody has been tested in immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AG

Positive Control :

Synonyms :

Nrxn1, Neurexin I-alpha

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NRXN1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ANR-031), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage