antibody

Anti-Neuropeptide S Receptor (extracellular) Antibody

ANR-029

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry

ANR-029-CF_0.2 ml

Anti-Neuropeptide S Receptor (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

Q8BZP8

IFC IHC LCI WB

Human Rat Mouse

Rabbit

BLP-NR029

Rat - 15 out of 16 amino acid residues identical, Human - 14 out of 16 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Extracellular, 3rd loop.

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)DNFNVLPDTKERFYAS, corresponding to amino acid residues 297 - 312 of mouse NPSR1

The Neuropeptide S Receptor (NPSR1), also known as GPR154, GPRA, or VRR1, belongs to the G protein-coupled receptor (GPCR) superfamily and is activated by neuropeptide S (NPS)1,2.NPS is a 20-residue peptide, named due to the presence of a serine (S) residue in the N-terminal position of the molecule. NPS expression is limited to a few discrete brain areas, such as in isolated cells of the amygdala, the hypothalamus, and specific regions of the brainstem. The primary sequence of NPS is highly conserved among vertebrates especially at the N-terminus, which is crucial for biological activity. The positions 2, 3, 4, and 5 on N-terminus of NPS play important roles in bioactivity and activation of NPSR11,2.The Neuropeptide S Receptor is widely distributed in the brain particularly in regions that are associated with regulation of stress response, memory, the olfactory system, and regulation of arousal. NPSR1 can signal via both Gq and Gs to increase cellular excitability and inhibit cell growth. Via the Gq pathway, NPSR1 leads to activation of Gq-phospholipase C and cleavage of phospholipid phosphatidylinositol 4,5-bisphosphate to inositol 1,4,5-trisphosphate (IP3) and diacylglycerol. IP3 activates IP3 receptor and releases Ca2 + from the ER. In some neurons, store-operated calcium channels are also activated, allowing entry of extracellular calcium into the cells 2.In the Gs pathway, NPSR signaling activates adenylate cyclase, increased synthesis of cAMP, and activation of protein kinase A 2.NPSR1 activation, like other GPCRs, also leads to activation of extracellular signal-regulated kinase 1/2 (ERK1/2) 2. Knockout studies in mice demonstrated that the NPS system produces anti-aggressive effects, reduces social anxiety behavior, modulates fear response and enhances motor performance skills3,4. A growing body of evidence suggests a close relationship between neuropeptide S and its receptor NPSR1 system in Parkinson's disease (PD). Indeed, administration of NPS to the central nervous syatem, ameliorates motor and non-motor dysfunctions in PD models, suggesting that targeting the NPS/NPSR1 system might be beneficial for Parkinson-related signs and symptoms 5.

IFC IHC LCI WB

Alomone Labs

NPSR1, G-Protein Coupled Receptor 154, GPR154, G-Protein Coupled Receptor for Asthma Susceptibility, GPRA

A Rabbit Polyclonal antibody to Neuropeptide S Receptor (extracellular)

Anti-Neuropeptide S Receptor (extracellular) Antibody (#ANR-029) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize NPSR1 from mouse, rat and human samples.

BLP-NR029

NA

NPSR1, G-Protein Coupled Receptor 154, GPR154, G-Protein Coupled Receptor for Asthma Susceptibility, GPRA

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

NPSR1

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:400

1:200