Anti-NPY5R Antibody | Alomone Labs ANR-025 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NPY5R Antibody

catalog :

ANR-025

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membrane: - 1. Anti-NPY5R Antibody (#ANR-025), (1:200).2. Anti-NPY5R Antibody, preincubated with NPY5R Blocking Peptide (#BLP-NR025).

image 2 :

Expression of neuropeptide Y5 receptor in rat striatum - Immunohistochemical staining of rat striatum using Anti-NPY5R Antibody (#ANR-025). A. NPY5R (red) appears in the striatal matrix (asterisk) and in nerve cells in the matrix (vertical arrows). B. Parvalbumin (green) appears in the striatal matrix. C. Merge of NPY5R and parvalbumin demonstrates co-localization in a sub-set of cells (horizontal arrows). DAPI is used as the counterstain (blue).

image 3 :

Expression of neuropeptide Y5 receptor in rat DRG - Immunocytochemical staining of a primary culture of rat dorsal root ganglion (DRG) neurons. A D. A paraformaldehyde-fixed and permeabilized DRG primary culture was stained with Anti-NPY5R Antibody(#ANR-025) (1:100) followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody. B E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B. F. Merged images ofpanels D and E.Magnification:A-C: x20E-F: x100

product information

CAT :

ANR-025

SKU :

ANR-025-CF_0.2 ml

Product Name :

Anti-NPY5R Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63634

Applications :

IC IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR025

Homology :

Mouse, bovine, canis - identical; human - 15/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

SFIRKHRRRYSKKTAC, corresponding to amino acid residues 291-306 of rat NPY5R

Product Page - Scientific background :

Neuropeptide Y (NPY) is a 36 amino acid peptide neurotransmitter present in the central and peripheral nervous systems.1 NPY is involved in the regulation of a broad range of functions such as eating behavior, hypertension, and reproduction.2NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R), and Y6 (NPY6R).2 Four of these (Y1, Y2, Y4, and Y5) are expressed in humans.2 The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Activation of these receptors by NPY affects [Ca2+]i, adenylate cyclase signalling, and a number of ion channel functions.3 Based on their high frequency and density in steroid hormone-producing tumors, the NPYRs might be of potential use in tumor management.2NPY5R as well as NPY1R and NPY2R are expressed in the hypothalamus, in the brain stem, and in peripheral tissues, such as blood vessels, lung, kidney, adrenal glands, stomach, colon, heart, pancreas, and intestine. NPY5R has been postulated as the mediator of the feeding response to NPY and related peptides (125 in Kamiji, M. M). Its localization in the brain is consistent with this hypothesis.4

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Neuropeptide Y5 receptor, Neuropeptide Y receptor type 5, NPY Y5 receptor, NPYY5

Short Description :

A Rabbit Polyclonal Antibody to Neuropeptide Y5 Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat neuropeptide Y5 receptor (NPY5R). Anti-NPY5R Antibody (#ANR-025) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize NPY5R from rat, human and mouse samples.

Negative Control :

BLP-NR025

Positive Control :

Synonyms :

Neuropeptide Y5 receptor, Neuropeptide Y receptor type 5, NPY Y5 receptor, NPYY5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NPY5R

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage