Anti-NPY4R Antibody | Alomone Labs ANR-024 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NPY4R Antibody

catalog :

ANR-024

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:500; loading ...; fig 3	Urata Y, Salehi R, Lima P, Osuga Y, Tsang B. Neuropeptide Y regulates proliferation and apoptosis in granulosa cells in a follicular stage-dependent manner. J Ovarian Res. 2020;13:5 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membrane (lanes 1 and 4), cortex (lanes 2 and 5), and hippocampus (lanes 3 and 6) lysates: - 1,2,3. Anti-NPY4R Antibody (#ANR-024), (1:200).4,5,6. Anti-NPY4R Antibody, preincubated with NPY4R Blocking Peptide (#BLP-NR024).

image 2 :

Peptide NINFKKDIKALVLTC corresponding to amino acid residues 326-340 of rat NPY4R (AccessionQ63447). Intracellular C-terminus.

image 3 :

Expression of neuropeptide Y4 receptor in ratDRG - Immunocytochemical staining of a primary culture of rat dorsal root ganglion(DRG) neurons. A D.A paraformaldehyde-fixed and permeabilized DRG primary culture was stained with Anti-NPY4R Antibody(#ANR-024) (1:100) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. B E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of A and B. F.Merged images of D and E.Magnification:A-C: x20.E-F: x100.

product information

CAT :

ANR-024

SKU :

ANR-024-CF_0.2 ml

Product Name :

Anti-NPY4R Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63447

Applications :

IC IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR024

Homology :

Mouse - identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

NINFKKDIKALVLTC corresponding to amino acid residues 326-340 of rat NPY4R

Product Page - Scientific background :

Neuropeptide Y (NPY) is a 36 amino acid peptide neurotransmitter in the central (CNS) and peripheral nervous systems.1 It belongs to the NPY family, along with peptide YY (PYY) and pancreatic polypeptide (PP). NPY is involved in the regulation of a broad range of homeostatic functions such as eating behavior, hypertension, and reproduction.2NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R), and Y6 (NPY6R).2 Four of these (Y1, Y2, Y4, and Y5) are expressed in humans.2The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Activation of these receptors by NPY produces effects on [Ca2+]i, adenylate cyclase, and a number of ion channels.3 Based on their high frequency and density in steroid hormone-producing tumors, the NPYRs might be of potential use in tumor management.2NPY4R preferentially binds PP, while NPY1R, NPY2R, and NPY5R bind both NPY and PP.NPY1R, NPY2R, and NPY5R share a low degree of identity (27-31% overall). NPY4R is more similar to NPY1R, displaying an overall identity of 43-53%.4Human NPY4R (also named pancreatic polypeptide receptor 1) mRNA is highly expressed in the small and large intestines and the prostate. Tissue distribution studies in human and mouse suggest potential roles for NPY4R in the gastrointestinal tract, heart, and prostate, as well as in neural and endocrine signaling. Only low levels of NPY4R expression in various areas of brain were displayed.5

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Neuropeptide Y4 receptor, Neuropeptide Y receptor type 4, Pancreatic polypeptide receptor 1, PPYR1

Short Description :

A Rabbit Polyclonal Antibody to Neuropeptide Y4 Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat neuropeptide Y4 receptor (NPY4R). Anti-NPY4R Antibody (#ANR-024) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize NPY4R from human, rat, and mouse samples.

Negative Control :

BLP-NR024

Positive Control :

Synonyms :

Neuropeptide Y4 receptor, Neuropeptide Y receptor type 4, Pancreatic polypeptide receptor 1, PPYR1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NPY4R

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage