Anti-NPY2R Antibody | Alomone Labs ANR-022 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NPY2R Antibody

catalog :

ANR-022

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:500; loading ...; fig 3	Urata Y, Salehi R, Lima P, Osuga Y, Tsang B. Neuropeptide Y regulates proliferation and apoptosis in granulosa cells in a follicular stage-dependent manner. J Ovarian Res. 2020;13:5 pubmed publisher
	Darche F, Rivinius R, Rahm A, Köllensperger E, Leimer U, Germann G, et al. In vivo cardiac pacemaker function of differentiated human mesenchymal stem cells from adipose tissue transplanted into porcine hearts. World J Stem Cells. 2020;12:1133-1151 pubmed publisher

image

image 1 :

Western blot analysis of rat hippocampus (lanes 1 and 3) and rat whole brain lysates (lanes 2 and 4): - 1,2. Anti-NPY2R Antibody (#ANR-022), (1:200).3,4. Anti-NPY2R Antibody, preincubated with NPY2R Blocking Peptide (#BLP-NR022).

image 2 :

Expression of neuropeptide Y2 receptor in rat cerebellum - Immunohistochemical staining of frozen section of rat cerebellum using Anti-NPY2R Antibody (#ANR-022), (1:100). NPY2R (red) is detected in Purkinje cells. B. Nuclei were stained using DAPI as the counterstain. C. Merged image of A and B.

image 3 :

Expression of neuropeptide Y2receptor in rat cerebellum - Immunohistochemical staining of frozen section of rat cerebellum using Anti-NPY2R Antibody(#ANR-022) (1:100). NPY2R(red)is detected in Purkinje cells. B. Nuclei were stained using DAPI as the counterstain. C. Merged image of A and B.

product information

CAT :

ANR-022

SKU :

ANR-022-CF_0.2 ml

Product Name :

Anti-NPY2R Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P97295

Applications :

IC IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR022

Homology :

Rat - identical; human - 18/19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

CEQRLDAIHSEVSMTFKAK, corresponding to amino acid residues 346-364 of mouse NPY2R

Product Page - Scientific background :

Neuropeptide Y (NPY) is a 36-amino acid peptide neurotransmitter in the central (CNS) and peripheral nervous systems.1 It belongs to the NPY family, together with peptide YY (PYY) and pancreatic polypeptide (PP). NPY is involved in regulation of a broad range of homeostatic functions such as eating behavior, hypertension, and reproduction.2NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R), and Y6 (NPY6R).2 Four of these, Y1, Y2, Y4, and Y5, are expressed in humans.2The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Activation of these receptors by NPY produces effects on [Ca2+]i, adenylate cyclase, and a number of ion channels.3 Based on the high frequency and density of NPY receptors in steroid hormone-producing tumors, the NPY receptors might be of potential use in tumor management.2NPY2R is primarily expressed in neurons as a presynaptic receptor. It is also strongly expressed in epithelia of the visceral tissues, including colon and kidney.4 NPY2R is involved in inhibition of neurotransmitter release mediated by NPY.5 Release of glutamate, the principal neuroexcitatory transmitter, is inhibited via the NPY2R receptor.6 Involvement of NPY2R in feeding behavior was also reported. PYY(3-36), an NPY2R agonist, acts to inhibit feeding via NPY2R.7

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Neuropeptide Y2 receptor, Neuropeptide Y receptor type 2, NPY Y2 receptor

Short Description :

A Rabbit Polyclonal Antibody to Neuropeptide Y2 Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the mouse neuropeptide Y2 receptor (NPY2R). Anti-NPY2R Antibody (#ANR-022) can be used in western blot analysis, immunohistochemistry, and immunocytochemical applications. It has been designed to recognize NPY2R from human, rat, and mouse samples.

Negative Control :

BLP-NR022

Positive Control :

Synonyms :

Neuropeptide Y2 receptor, Neuropeptide Y receptor type 2, NPY Y2 receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NPY2R

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage