Anti-NPY1R Antibody | Alomone Labs ANR-021 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NPY1R Antibody

catalog :

ANR-021

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Hoang J, Salavatian S, Yamaguchi N, Swid M, David H, Vaseghi M. Cardiac sympathetic activation circumvents high-dose beta blocker therapy in part through release of neuropeptide Y. JCI Insight. 2020;5: pubmed publisher

image

image 1 :

Western blot analysis of human Jurkat acute T cell leukemia (lanes 1 and 4), human K562 chronic myelogeneous leukemia (lanes 2 and 5), and rat RBL basophilic leukemia (lanes 3 and 6) cell lysates and rat brain lysates (lanes 7 and 8): - 1,2,3,7. Anti-NPY1R Antibody (#ANR-021), (1:200).4,5,6,8. Anti-NPY1R Antibody, preincubated with NPY1R Blocking Peptide (#BLP-NR021).

image 2 :

Expression of neuropeptide Y1 receptor in rat striatum - Immunohistochemical staining of rat striatum using Anti-NPY1R Antibody (#ANR-021). A. NPY1R (red) appears in the striatal matrix (white asterisk) and in medium-size cells in the matrix (arrows). B. Parvalbumin (green) appears in the striatal matrix. C. Confocal merge of NPY1R and parvalbumin indicates that NPY1R is restricted to granule cells. DAPI is used as the counterstain (blue).

image 3 :

Expressionof neuropeptide Y1 receptor in DRG neurons - Immunocytochemical staining of a primary culture of rat dorsal root ganglion (DRG) neurons. A D. A paraformaldehyde-fixed and permeabilized DRG primary culture was stained with Anti-NPY1R Antibody(#ANR-021) (1:100) followed by Alexa-555-conjugated goat-anti-rabbit secondary antibody. B E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of A and B. F.Merged images of D and E.Magnification:A-C: x20E-F: x100

product information

CAT :

ANR-021

SKU :

ANR-021-CF_0.2 ml

Product Name :

Anti-NPY1R Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P25929

Applications :

IC IF IHC WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR021

Homology :

Rat, mouse - 14/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)RLKRRNNMMDKMRDNK, corresponding to amino acid residues 237-252 of human NPY1R

Product Page - Scientific background :

Neuropeptide Y (NPY) is a 36-amino acid peptide neurotransmitter in the central (CNS) and peripheral nervous systems.1 It belongs to the NPY family, together with the peptide YY (PYY) and the pancreatic polypeptide (PP). NPY is involved in regulation of a broad range of homeostatic functions such as eating behavior, hypertension, and reproduction.2NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R) and Y6 (NPY6R).2 Four of these, Y1, Y2, Y4, and Y5, are expressed in humans.2 The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Activation of these receptors by NPY produces effects on [Ca2+]i, adenylate cyclase, and a number of ion channels.3 Based on the high frequency and density of NPY receptors in steroid hormone-producing tumors, the NPY receptors might be of potential use in tumor management.2NPY1R is considered to be a postsynaptic receptor. In the CNS, NPY1Rs are concentrated in the cortical areas of the brain, cerebral cortex, olfactory nucleus, hippocampus, hypothalamic nuclei, and thalamic nuclei.4 In addition, this receptor has a bimodal role in the immune system, serving as a strong negative regulator on T cells as well as a key activator of antigen-presenting cell function.5

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Neuropeptide Y1 receptor, Neuropeptide Y receptor type 1, NPYR, NPYY1

Short Description :

A Rabbit Polyclonal Antibody to Neuropeptide Y1 Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the human neuopeptide Y1 receptor (NPY1R). Anti-NPY1R Antibody (#ANR-021) can be used in western blot analysis, immunohistochemistry, and immunocytochemical applications. It has been designed to recognize NPY1R from human, rat, and mouse samples.

Negative Control :

BLP-NR021

Positive Control :

Synonyms :

Neuropeptide Y1 receptor, Neuropeptide Y receptor type 1, NPYR, NPYY1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NPY1R

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage