Anti-GAP43 Antibody | Alomone Labs ANR-016 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GAP43 Antibody

catalog :

ANR-016

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2 Anti-GAP43 Antibody (#ANR-016), (1:400).3,4. Anti-GAP43 Antibody, preincubated with GAP43 Blocking Peptide (#BLP-NR016).

image 2 :

Expression of GAP43 in rat hippocampus - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GAP43 Antibody (#ANR-016), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. GAP43 staining (green) appears in a layer bordering granule layer (G), (arrow). B. Calbindin D28k staining (red) is detected in granule layer (G) neurons. C. Merge of the two images demonstrates distinct spatial localizations in the hippocampal dentate gyrus. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANR-016

SKU :

ANR-016-CF_0.2 ml

Product Name :

Anti-GAP43 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P07936

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR016

Homology :

Mouse - identical; human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KPKESARQDEGKED, corresponding to amino acid residues 205-218 of rat GAP43

Product Page - Scientific background :

Growth associated protein 43 (GAP43) is a neuron specific phosphoprotein that plays an important role in the process of nerve growth, development, regeneration and axonal remodeling. GAP43 belongs to the calmodulin-binding protein family and is highly distributed in the presynaptic membrane1.GAP43 is expressed in developing neurons, especially in the growth cone. In mature neurons, GAP43 is detected in regenerating nerves. In brain injury, neurons exhibit sprouting of the lateral branches and responsive regeneration of the axon accompanied by increased expression of GAP43. GAP43 expression is used as an important tool for evaluating axon injury and the regenerative response in the mature central nervous system (CNS)1,2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Axonal membrane protein GAP-43, Growth-associated protein 43, Neuromodulin, Neural phosphoprotein B-50

Short Description :

A Rabbit Polyclonal Antibody to GAP43

Long Description :

Anti-GAP43 Antibody (#ANR-016) is a highly selective antibody directed against an epitope of rat Neuromodulin. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GAP43 from human, mouse, and rat samples.

Negative Control :

BLP-NR016

Positive Control :

Synonyms :

Axonal membrane protein GAP-43, Growth-associated protein 43, Neuromodulin, Neural phosphoprotein B-50

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GAP43

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage