Guinea Pig Anti-GAP43 Antibody | Alomone Labs ANR-016-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea Pig Anti-GAP43 Antibody

catalog :

ANR-016-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):

image 2 :

Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:

image 3 :

Expression of GAP43 in rat hippocampus.

product information

CAT :

ANR-016-GP

SKU :

ANR-016-GP-CF_0.2 ml

Product Name :

Guinea Pig Anti-GAP43 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P07936

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-NR016

Homology :

Mouse - identical; human - 13 out of 14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KPKESARQDEGKED, corresponding to amino acid residues 205-218 of rat GAP43

Product Page - Scientific background :

Growth associated protein 43 (GAP43) is a neuron specific phosphoprotein that plays an important role in the process of nerve growth, development, regeneration and axonal remodeling. GAP43 belongs to the calmodulin-binding protein family and is highly distributed in the presynaptic membrane1.GAP43 is expressed in developing neurons, especially in the growth cone. In mature neurons, GAP43 is detected in regenerating nerves. In brain injury, neurons exhibit sprouting of the lateral branches and responsive regeneration of the axon accompanied by increased expression of GAP43. GAP43 expression is used as an important tool for evaluating axon injury and the regenerative response in the mature central nervous system (CNS)1,2.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Axonal membrane protein GAP-43, Growth-associated protein 43, Neuromodulin, Neural phosphoprotein B-50

Short Description :

A Guinea Pig Polyclonal Antibody to GAP43

Long Description :

Guinea Pig Anti-GAP43 Antibody (#ANR-016-GP) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GAP43 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-GAP43 Antibody (#ANR-016) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Negative Control :

BLP-NR016

Positive Control :

Synonyms :

Axonal membrane protein GAP-43, Growth-associated protein 43, Neuromodulin, Neural phosphoprotein B-50

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GAP43

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage