Anti-Synapsin I (SYN1) Antibody | Alomone Labs ANR-014 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Synapsin I (SYN1) Antibody

catalog :

ANR-014

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain synaptosomal fractions: - 1,2. Anti-Synapsin I (SYN1) Antibody (#ANR-014), (1:400).3,4. Anti-Synapsin I (SYN1) Antibody, preincubated with Synapsin I/SYN1 Blocking Peptide (#BLP-NR014).

image 2 :

Expression of Synapsin-1 in mouse cerebellum - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Synapsin I (SYN1) Antibody (#ANR-014), (1:400), followed by anti-rabbit-Alexa-488 antibody. Synapsin-1 staining (green) appears in Purkinje cell bodies (horizontal arrows) and in segments of the dendritic tree (vertical arrows). Nuclei are stained with DAPI (blue).

product information

CAT :

ANR-014

SKU :

ANR-014-CF_0.2 ml

Product Name :

Anti-Synapsin I (SYN1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P09951

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR014

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, region C

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RLHKKLGTEEFP(G), corresponding to amino acid residues 234 - 245 of rat Synapsin-1

Product Page - Scientific background :

Synapsins are a family of synaptic vesicle associated phosphoproteins consisting of three members: SYN1, SYN2, and SYN3, with several alternative splicing leading to 10 different isoforms. They are involved in synaptic transmission and plasticity, several stages of neurodevelopment, such as axon outgrowth and synapse formation. Synapsins are localized in presynaptic nerve terminals of central neurons1.Synapsin I is a peripheral membrane protein of synaptic vesicles that is phosphorylated at least at three different sites by various protein kinases. All synapsins have a single phosphorylation site in the short N-terminal domain A (site 1) that regulates neurite elongation and synaptic vesicle mobilization2.Synapsin I exhibits an asymmetric structure and exists in two forms, designated synapsin Ia and synapsin Ib. The primary structure of synapsin I is highly conserved, 95% of the protein's residues are invariant and contain two major structural domains. The protein is divided into a hydrophobic, collagenase resistant head region and a proline rich, collagenase sensitive tail3,4. Synapsin I has been suggested to play an important role in the regulation of neurotransmitter release and the organization of cytoskeletal organization including actin filaments, microtubules and spectrin in the presynaptic terminal5.In vitro studies show that the dephospho-form of synapsin I binds synaptic vesicles, binds and bundles F-actin, and promotes G actin polymerization5.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Synapsin-1

Short Description :

A Rabbit Polyclonal Antibody to Synapsin I

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Synapsin I. Anti-Synapsin I (SYN1) Antibody (#ANR-014) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Synapsin I from rat, mouse and human samples.

Negative Control :

BLP-NR014

Positive Control :

Synonyms :

Synapsin-1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SYN1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage