Anti-Synaptophysin Antibody | Alomone Labs ANR-013 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Synaptophysin Antibody

catalog :

ANR-013

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:2000; loading ...; fig 4c	Yang P, Leu D, Ye K, Srinivasan C, Fike J, Huang T. Cognitive impairments following cranial irradiation can be mitigated by treatment with a tropomyosin receptor kinase B agonist. Exp Neurol. 2016;279:178-186 pubmed publisher
	Hsu P, Ma Y, Fang Y, Huang J, Gan Y, Chang P, et al. Cullin 7 mediates proteasomal and lysosomal degradations of rat Eag1 potassium channels. Sci Rep. 2017;7:40825 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat PC12 pheochromocytoma cell line lysate (lanes 3 and 6): - 1-3. Anti-Synaptophysin Antibody (#ANR-013), (1:400).4-6. Anti-Synaptophysin Antibody, preincubated with Synaptophysin Blocking Peptide (#BLP-NR013).

image 2 :

Expression of Synaptophysin in rat DRG - Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-Synaptophysin Antibody (#ANR-013), (1:100). SYP (red) is expressed in DRG neurons. Hoechst 33342 (blue) shows nuclear staining and is used as the counterstain.

image 3 :

Expression of Synaptophysin in rat DRG - Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections usingAnti-SynaptophysinAntibody (#ANR-013) (1:100). SYP (red) is expressed in DRG neurons. Hoechst 33342 (blue) shows nuclear staining and is used as the counterstain.

product information

CAT :

ANR-013

SKU :

ANR-013-CF_0.2 ml

Product Name :

Anti-Synaptophysin Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P07825

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR013

Homology :

Rat, mouse, human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd intravesicular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CRQTGNT(S)KELRD, corresponding to amino acid residues 178-190 of rat synaptophysin

Product Page - Scientific background :

Synaptophysin is the major integral membrane protein of small synaptic vesicles1-3. It belongs to a family of proteins which includes synaptogyrin and synaptoporin. The protein contains 4 transmembrane domains and cytoplasmic N- and C-termini4. There are two intravesicular loops that contain disulfide bonds. The protein is N-glycosylated on the first intravesicular loop, a modification important for the synaptic vesicle targeting of synaptophysin. Indeed, a mutant form of the protein, unable to undergo glycolysation, leads to the accumulation of the protein in the cell body of neurons indicating that N-glycosylation is required for the synaptic localization of synaptophysin5. The C-terminal tail is extensively phosphorylated on Tyr residues. However, the biological significance of the post-translational modification is not yet determined6.Although exact and specific role of synaptophysin remains a mystery, a study suggests that the protein is required for efficient endocytosis of synaptic vesicles in cultured hippocampal neurons6.Since synaptophysin is exclusively detected in synaptic vesicles, it is widely used as a marker for presynaptic vesicles.Genetic screening in humans and behavioral studies in rodents show that loss of synaptophysin expression or expression of a truncated form of the protein may be attributed to mental retardation and/or learning deficits7,8. Interestingly though, synaptic transmission in synaptophysin knockout mice remains normal6.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Major synaptic vesicle protein p38, SYP

Short Description :

A Rabbit Polyclonal Antibody to Synaptophysin

Long Description :

Anti-Synaptophysin Antibody (#ANR-013) is a highly specific antibody directed against an epitope of the rat protein. It can be used in western blot and immunohistochemistry applications. It has been designed to recognize synaptophysin from mouse, rat, and human samples.

Negative Control :

BLP-NR013

Positive Control :

Synonyms :

Major synaptic vesicle protein p38, SYP

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SYP

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage