antibody

Guinea Pig Anti-Synaptophysin Antibody

ANR-013-GP

polyclonal

guinea-pigs

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry

ANR-013-GP-CF_0.2 ml

Guinea Pig Anti-Synaptophysin Antibody

Antibodies

Antibodies

Polyclonal

P07825

IHC WB

Human Rat Mouse

Guinea Pig

BLP-NR013

Rat, mouse, human - 12 out of 13 amino acid residues identical

PBS pH7.4

Guinea pig total IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

2nd intravesicular loop

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

CRQTGNT(S)KELRD, corresponding to amino acid residues 178-190 of rat synaptophysin

Synaptophysin is the major integral membrane protein of small synaptic vesicles1-3. It belongs to a family of proteins which includes synaptogyrin and synaptoporin. The protein contains 4 transmembrane domains and cytoplasmic N- and C-termini4. There are two intravesicular loops that contain disulfide bonds. The protein is N-glycosylated on the first intravesicular loop, a modification important for the synaptic vesicle targeting of synaptophysin. Indeed, a mutant form of the protein, unable to undergo glycolysation, leads to the accumulation of the protein in the cell body of neurons indicating that N-glycosylation is required for the synaptic localization of synaptophysin5. The C-terminal tail is extensively phosphorylated on Tyr residues. However, the biological significance of the post-translational modification is not yet determined6.Although exact and specific role of synaptophysin remains a mystery, a study suggests that the protein is required for efficient endocytosis of synaptic vesicles in cultured hippocampal neurons6.Since synaptophysin is exclusively detected in synaptic vesicles, it is widely used as a marker for presynaptic vesicles.Genetic screening in humans and behavioral studies in rodents show that loss of synaptophysin expression or expression of a truncated form of the protein may be attributed to mental retardation and/or learning deficits7,8. Interestingly though, synaptic transmission in synaptophysin knockout mice remains normal6.

IHC WB

Alomone Labs

Major synaptic vesicle protein p38, SYP

A Guinea Pig Polyclonal Antibody to Synaptophysin

Guinea Pig Anti-Synaptophysin Antibody (#ANR-013-GP, formerly AGP-144) is a highly specific antibody directed against an epitope of the rat Synaptophysin protein. The antibody can be used in western blot analysis and immunohistochemistry applications. It has been designed to recognize Synaptophysin from rat, mouse and human samples. The antigen used to immunize guinea pigs is the same as Anti-Synaptophysin Antibody (#ANR-013) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

BLP-NR013

NA

Major synaptic vesicle protein p38, SYP

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

SYP

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:200

1:200