Anti-CPLX1/2 Antibody | Alomone Labs ANR-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CPLX1/2 Antibody

catalog :

ANR-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2. Anti-CPLX1/2 Antibody (#ANR-011), (1:200).3,4. Anti-CPLX1/2 Antibody, preincubated with CPLX1/2 Blocking Peptide (#BLP-NR011).

product information

CAT :

ANR-011

SKU :

ANR-011-CF_0.2 ml

Product Name :

Anti-CPLX1/2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P63041, P84087

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR011

Homology :

Complexin 1: Mouse, human - identicalComplexin 2: Rat, mouse, human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KDMGKMLGGDEEKD, corresponding to amino acid residues 14-27 of rat Complexin-1

Product Page - Scientific background :

Complexins (1 through 4) are small cytosolic proteins that bind tightly to a complex of SNARE proteins to regulate synaptic vesicle membrane fusion via its interaction with synaptotagmin. Complexins activate the quick release of neurotransmitters, triggered by calcium release. They also regulate the "spontaneous release" of neurotransmitters1-3.Complexins are evolutionarily conserved in mammals, Drosophila, and Caenorhabditis elegans1,2. They are divided into four structural domains: flexible N- and C-terminal domains, an accessory domain, and a central α-helical domain. The N-terminal domain plays a role in activation of fast synchronous release in murine neurons and in isolated chromaffin cells. The C-terminal domain binds to phospholipids and is important for regulating spontaneous release in neuronal cultures and suppressing Ca2+-independent fusion in vitro. The accessory domain is necessary for regulating spontaneous release and the central domain is required for all functions1,2.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Complexin 1, Complexin I, Synaphin-2, Complexin 2, Complexin II, Synaphin-1

Short Description :

A Rabbit Polyclonal Antibody to Complexin-1 and Complexin-2

Long Description :

Anti-CPLX1/2 Antibody (#ANR-011) is an antibody directed against epitopes of rat Complexin 1 and Complexin 2. The antibody can be used in western blot analysis. It has been designed to recognize both Complexin 1 and Complexin 2 from human, rat, and mouse samples.

Negative Control :

BLP-NR011

Positive Control :

Synonyms :

Complexin 1, Complexin I, Synaphin-2, Complexin 2, Complexin II, Synaphin-1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CPLX1, CPLX2

AB Specifictiy :

The antibody will recognize both Complexin-1 and Complexin-2.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage