Anti-Syntaxin 2 Antibody | Alomone Labs ANR-008 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Syntaxin 2 Antibody

catalog :

ANR-008

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Satoh K, Narita T, Katsumata Kato O, Sugiya H, Seo Y. Involvement of myristoylated alanine-rich C kinase substrate phosphorylation and translocation in cholecystokinin-induced amylase release in rat pancreatic acini. Am J Physiol Gastrointest Liver Physiol. 2016;310:G399-409 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-Syntaxin 2 Antibody (#ANR-008), (1:600).2. Anti-Syntaxin 2 Antibody, preincubated with Syntaxin 2 Blocking Peptide (#BLP-NR008).

image 2 :

Western blot analysisof rat brain membranes: - 1.Anti-Syntaxin 2Antibody (#ANR-008) (1:600).2.Anti-Syntaxin 2 Antibody preincubated with the control peptide antigen.

product information

CAT :

ANR-008

SKU :

ANR-008-CF_0.2 ml

Product Name :

Anti-Syntaxin 2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P50279

Applications :

IF IHC WB

Reactivity :

Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR008

Homology :

Human - 16/17 amino acid residues identical; mouse - 15/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

RDRLPDLTACRKSDDGDN, corresponding to amino acid residues 2-19 of rat Syntaxin 2

Product Page - Scientific background :

Syntaxin 2 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60-70 amino acids that are arranged in heptad repeats1,2.SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.Syntaxin 2, like most SNAREs, is a type IV membrane protein with a relatively large N-terminus containing the SNARE motif located in the cytoplasmic side and a transmembrane domain located close to the C-terminus that functions as an anchor1,2.Syntaxin 2 functions as a plasma membrane protein t-SNARE, which together with vesicular v-SNARE proteins is involved in regulated exocytosis in cells such as platelets, sperm cells and pancreatic acinar cells3-5. Intriguingly, Syntaxin 2 has been also identified as epimorphin, an extracellular membrane protein implicated in branching morphogenesis, a developmental process essential for the formation of many organs, including mammary gland, lung, kidney, and salivary gland6.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

STX2, Epimorphin, EPIM

Short Description :

A Rabbit Polyclonal Antibody to Syntaxin 2

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Syntaxin 2. Anti-Syntaxin 2 Antibody (#ANR-008) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Syntaxin 2 from rat, mouse, and human samples.

Negative Control :

BLP-NR008

Positive Control :

Synonyms :

STX2, Epimorphin, EPIM

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

STX2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:600

more info or order :

Alomone Labs product webpage