Anti-Syntaxin 3 Antibody | Alomone Labs ANR-005 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Syntaxin 3 Antibody

catalog :

ANR-005

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Ojeda Naharros I, Gesemann M, Mateos J, Barmettler G, Forbes A, Ziegler U, et al. Loss-of-function of the ciliopathy protein Cc2d2a disorganizes the vesicle fusion machinery at the periciliary membrane and indirectly affects Rab8-trafficking in zebrafish photoreceptors. PLoS Genet. 2017;13:e1007150 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 3) and kidney (lanes 2 and 4) membranes: - 1,2. Anti-Syntaxin 3 Antibody (#ANR-005), (1:200).3,4. Anti-Syntaxin 3 Antibody, preincubated with Syntaxin 3 Blocking Peptide (#BLP-NR005).

image 2 :

Western blot analysis of rat brain (lanes 1 and 3) and kidney (lanes 2 and 4) membranes: - 12.Anti-Syntaxin 3Antibody (#ANR-005) (1:200).34. Anti-Syntaxin 3 Antibody preincubated with the control peptide antigen.

image 3 :

Expression of GPR65 in rat fornix - Immunohistochemical staining of immersion-fixed free floating rat frozen brain sections usingAnti-GPR65 (TDAG8) (extracellular)Antibody (#AGR-043) (1:1200) followed by goat-anti-rabbit-AlexaFluor-488. GPR65 staining (green) appears in glial processes (vertical arrows) and in the side of fornix (FX) facing the ventricle (V horizontal arrow). Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANR-005

SKU :

ANR-005-CF_0.2 ml

Product Name :

Anti-Syntaxin 3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q08849

Applications :

IC IF WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR005

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Cytoplasmic, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

KDRLEQLKAKQLTQD(C), corresponding to amino acid residues 2-16 of rat syntaxin 3

Product Page - Scientific background :

Syntaxin 3 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60-70 amino acids that are arranged in heptad repeats1,2. SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.Syntaxin 3, like most SNAREs, is a type IV membrane protein with a relatively large N-terminus containing the SNARE motif located in the cytoplasmic side and a transmembrane domain located close to the C-terminus that functions as an anchor1,2.Syntaxin 3 functions as a plasma membrane protein t-SNARE that is involved in epithelial cell polarization. Indeed, Syntaxin 3 is specifically localized in the apical membrane of polarized epithelial cells where it likely has a role in the polarized trafficking of newly synthesized proteins3.

Applications may also work in :

IC IF WB

Supplier :

Alomone Labs

Target :

STX3

Short Description :

A Rabbit Polyclonal Antibody to Syntaxin 3

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Syntaxin 3. Anti-Syntaxin 3 Antibody (#ANR-005) can be used in western blot and immunocytochemistry applications. It has been designed to recognize Syntaxin 3 from rat, mouse and human samples.

Negative Control :

BLP-NR005

Positive Control :

Synonyms :

STX3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

STX3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage