Anti-Syntaxin 4 Antibody | Alomone Labs ANR-004 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Syntaxin 4 Antibody

catalog :

ANR-004

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Brasher M, Martynowicz D, Grafinger O, Hucik A, Shanks Skinner E, Uniacke J, et al. Interaction of Munc18c and syntaxin4 facilitates invadopodium formation and extracellular matrix invasion of tumor cells. J Biol Chem. 2017;292:16199-16210 pubmed publisher

image

image 1 :

Western blot anlysis of rat heart lysate: - 1. Anti-Syntaxin 4 Antibody (ANR-004), (1:400).2. Anti-Syntaxin 4 Antibody, preincubated with Syntaxin 4 Blocking Peptide (#BLP-NR004).

image 2 :

Expression of syntaxin 4 in rat pancreas - Immunohistochemical staining of rat pancreas paraffin embedded sections using Anti-Syntaxin 4 Antibody (#ANR-004), (1:100). STX4 (brown staining) is expressed in endocrine cells of the Isles of Langerhans (arrow). Hematoxilin is used as the counterstain.

image 3 :

Expression of syntaxin 4 in rat pancreas - Immunohistochemical staining of rat pancreas paraffin embedded sections usingAnti-Syntaxin 4Antibody (#ANR-004) (1:100). STX4 (brown staining) is expressed in endocrine cells of theIsles of Langerhans(arrow). Hematoxilin is used as the counterstain.

product information

CAT :

ANR-004

SKU :

ANR-004-CF_0.2 ml

Product Name :

Anti-Syntaxin 4 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q08850

Applications :

IHC IP WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR004

Homology :

Mouse - identical; human - 17/22 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

RDRTHELRQGDNISDDEDEVRV(C), corresponding to residues 2-23 of rat syntaxin 4

Product Page - Scientific background :

Syntaxin 4 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60-70 amino acids that are arranged in heptad repeats1,2.SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.Syntaxin 4, like most SNAREs, is a type IV membrane protein with a relatively large N-terminus containing the SNARE motif located in the cytoplasmic side and a transmembrane domain located close to the C-terminus that functions as an anchor1,2.Syntaxin 4 functions as a plasma membrane protein t-SNARE and has been shown to have an important role in pancreatic b-cell insulin secretion and in insulin-stimulated glucose uptake in skeletal muscle and adipose tissue by means of translocation of the glucose transporter GLUT4 from an intracellular compartment to the plasma membrane3,4.

Applications may also work in :

IHC IP WB

Supplier :

Alomone Labs

Target :

STX4, Renal carcinoma antigen NY-REN-31, P35-2

Short Description :

A Rabbit Polyclonal Antibody to Syntaxin 4

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Syntaxin 4. Anti-Syntaxin 4 Antibody (#ANR-004) can be used in western blot and immunohistochemical applications. It was designed to recognize Syntaxin 4 from rat, mouse and human samples.

Negative Control :

BLP-NR004

Positive Control :

Synonyms :

STX4, Renal carcinoma antigen NY-REN-31, P35-2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

STX4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage