Anti-Synaptotagmin-1 (SYT1) Antibody | Alomone Labs ANR-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Synaptotagmin-1 (SYT1) Antibody

catalog :

ANR-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Matta S, Moore Z, Walker F, Hill Yardin E, Crack P. An altered glial phenotype in the NL3R451C mouse model of autism. Sci Rep. 2020;10:14492 pubmed publisher
Peixoto Neves D, Soni H, Adebiyi A. Oxidant-induced increase in norepinephrine secretion from PC12 cells is dependent on TRPM8 channel-mediated intracellular calcium elevation. Biochem Biophys Res Commun. 2018;506:709-715 pubmed publisher
Haque F, Rancic V, Zhang W, Clugston R, Ballanyi K, Gosgnach S. WT1-Expressing Interneurons Regulate Left-Right Alternation during Mammalian Locomotor Activity. J Neurosci. 2018;38:5666-5676 pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2. Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003), (1:400).3,4. Anti-Synaptotagmin-1 (SYT1) Antibody, preincubated with Synaptotagmin-1/SYT1 Blocking Peptide (#BLP-NR003).

image 2 :

Expression of Synaptotagmin-I in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003), (1:400), followed by anti-rabbit-Alexa-488 antibody. Synaptotagmin-I staining (green) appears in the hilus of the hippocampal dentate gyrus. Nuclei are stained with DAPI (blue).

image 3 :

Western blot analysisof mouse (lanes 1 and 3) and rat (lanes 2 and 4)brain lysates: - 12.Anti-Synaptotagmin-1 (SYT1) Antibody(#ANR-003) (1:400).34. Anti-Synaptotagmin-1 (SYT1) Antibody preincubated with the control peptide antigen.

product information

CAT :

ANR-003

SKU :

ANR-003-CF_0.2 ml

Product Name :

Anti-Synaptotagmin-1 (SYT1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P21107

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR003

Homology :

Mouse - 18/19 amino acid residues identical; human, bovine - 17/20 amino acid residues identical; chicken - 15/19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intravesicular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

MVSASHPEALAAPVTTVAT(C), corresponding to amino acid residues 1-19 of rat synaptotagmin 1

Product Page - Scientific background :

Synaptotagmin 1 is a member of the Ca2+-binding synaptotagmin family of proteins that includes 16 members in vertebrates and is involved in regulated exocytosis and trafficking1,2.Synaptotagmins are type I membrane proteins located in intracellular vesicles and consist of a short N-terminal domain located in the vesicle lumen, a transmembrane domain and a cytosolic domain containing two Ca2+ binding domains1,2.Synaptotagmins are usually localized to distinct secretory vesicles and control their Ca2+-dependent fusion. Synaptotagmin I is predominantly expressed in neuronal cells and is an abundant constituent of synaptic vesicles1,2.Synaptotagmin I has a relatively low Ca2+ affinity and is able to bind Ca2+ mostly in the vicinity of Ca2+ channels pores. Indeed, Synaptotagmin I has been demonstrated to directly interact with selected Ca2+ channels. Synaptotagmin I also binds to the t-SNARE protein Syntaxin 1 in a Ca2+-dependent manner thus forming the prototypical Ca2+-dependent fast synaptic vesicle exocytosis protein complex that also includes SNAP-25 and VAMP1,2,3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Synaptotagmin I, SytI, p65, SVP65

Short Description :

A Rabbit Polyclonal Antibody to Synaptotagmin-1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against the intravesicular N-terminal domain of rat synaptotagmin 1. Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize synaptotagmin 1 from rat, mouse and human samples.

Negative Control :

BLP-NR003

Positive Control :

Synonyms :

Synaptotagmin I, SytI, p65, SVP65

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SYT1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage