Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody | Alomone Labs ANR-003-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody

catalog :

ANR-003-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysates: - 1. Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003-GP), (1:400). 2. Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003-GP), preincubated with Synaptotagmin-1 (SYT1) Blocking Peptide (#BLP-NR003).

image 2 :

Expression of Synaptotagmin-1 in rat cerebellum. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003-GP), (1:1000), followed by goat anti-guinea pig - Alexa Fluor-594. A. Synaptotagmin-1 immunoreactivity (red) appears in the granule layer (vertical arrows) and in the molecular layer (horizontal arrows). B. Pre-incubation of the antibody with Synaptotagmin-1 (SYT1) Blocking Peptide (#BLP-NR003), suppressed staining. Cell nuclei are stained with DAPI (blue). M = molecular layer, P = purkinje layer, G = granule layer.

product information

CAT :

ANR-003-GP

SKU :

ANR-003-GP-CF_0.2 ml

Product Name :

Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P21107

Applications :

IHC WB

Reactivity :

Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-NR003

Homology :

Mouse - 18 out of 19 amino acid residues identical, human, bovine - 17 out of 19 amino acid residues identical, chicken - 15 out of 19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intravesicular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

MVSASHPEALAAPVTTVAT(C), corresponding to amino acid residues 1-19 of rat synaptotagmin 1

Product Page - Scientific background :

Synaptotagmin 1 is a member of the Ca2+-binding synaptotagmin family of proteins that includes 16 members in vertebrates and is involved in regulated exocytosis and trafficking1,2.Synaptotagmins are type I membrane proteins located in intracellular vesicles and consist of a short N-terminal domain located in the vesicle lumen, a transmembrane domain and a cytosolic domain containing two Ca2+ binding domains1,2.Synaptotagmins are usually localized to distinct secretory vesicles and control their Ca2+-dependent fusion. Synaptotagmin I is predominantly expressed in neuronal cells and is an abundant constituent of synaptic vesicles1,2.Synaptotagmin 1 has a relatively low Ca2+ affinity and is able to bind Ca2+ mostly in the vicinity of Ca2+ channels pores. Indeed, Synaptotagmin 1 has been demonstrated to directly interact with selected Ca2+ channels. Synaptotagmin 1 also binds to the t-SNARE protein Syntaxin 1 in a Ca2+-dependent manner thus forming the prototypical Ca2+-dependent fast synaptic vesicle exocytosis protein complex that also includes SNAP-25 and VAMP1,2,3.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Synaptotagmin I, SytI, p65, SVP65

Short Description :

A Guinea Pig Polyclonal Antibody to Anti-Synaptotagmin-1 (SYT1)

Long Description :

Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003-GP) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Synaptotagmin-1 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Negative Control :

BLP-NR003

Positive Control :

Synonyms :

Synaptotagmin I, SytI, p65, SVP65

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SYT1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1000

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage