Anti-SNAP-25 Antibody | Alomone Labs ANR-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SNAP-25 Antibody

catalog :

ANR-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-SNAP-25 Antibody (#ANR-001), (1:1000).2. Anti-SNAP-25 Antibody, preincubated with SNAP-25 Blocking Peptide (BLP-NR001).

image 2 :

Cell surface detection of ANO6 in live human MEG-01 megakaryoblastic leukemia cell line: - ___Cells.___Cells + goat-anti-rabbit-FITC.___Cells +Anti-Anoctamin-6 (extracellular) Antibody (#ACL-016) 5g + goat-anti-rabbit-FITC.

product information

CAT :

ANR-001

SKU :

ANR-001-CF_0.2 ml

Product Name :

Anti-SNAP-25 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P60881

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NR001

Homology :

Human, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by DNA sequence and SDS-PAGE

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Cytoplasmic

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

VDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG, corresponding to the full-length of rat SNAP-25
MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEES
KDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNL
TDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQP
ARV
GST fusion protein with the sequence

Product Page - Scientific background :

SNAP-25 (synaptosomal-associated 25kD protein) is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60-70 amino acids that are arranged in heptad repeats1,2.SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.SNAP-25, unlike most SNAREs, lacks a membrane-spanning region, but is attached to the cytoplasmic side of the plasma membrane by posttranslational modifications such as palmitoylation or farnesylation1,2.SNAP-25 has been extensively studied for its role on neuronal and neuroendocrine cell exocytosis where it functions as one of the plasma membrane protein t-SNARE, which together with the vesicular v-SNARE protein VAMP and another t-SNARE such as Syntaxin 1, forms a trimeric, four-helical complex, which drives fusion of the two opposing bilayers1,2.SNAP-25 is the target of several botulinum neurotoxin (BoNT) types: type A, C and E. The neurotoxins cause specific proteolytic degradation of the SNAP-25 protein, which in turn causes SNARE complex disruption and inhibition of neurotransmitter release3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Synaptosomal-associated protein 25 kD

Short Description :

A Rabbit Polyclonal Antibody to SNAP-25

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against rat SNAP-25. Anti-SNAP-25 Antibody (#ANR-001) can be used in western blot and immunohistochemical applications. It has been designed to recognize SNAP-25 from rat, mouse and human samples.

Negative Control :

BLP-NR001

Positive Control :

Synonyms :

Synaptosomal-associated protein 25 kD

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SNAP25

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

3 µg fusion protein per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:1000

more info or order :

Alomone Labs product webpage