antibody

Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody

ANP-014

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry

ANP-014-CF_0.2 ml

Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

P14094

IHC WB

Human Rat Mouse

Rabbit

BLP-NP014

Rat, human - identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Extracellular, C-terminus.

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

CKAYGENIGYSEKDR, corresponding to amino acid residues 277 - 291 of mouse ATP1B1

Ion channels are multimeric proteins located in the plasma membrane that play a critical role in the regulation of cellular processes1. The gradient of sodium (Na+) and potassium (K+) across the plasma membrane is regulated by the Na+/K+-ATPase enzyme complex. Na/K-ATPase hydrolyzes one ATP molecule in order to pump three Na+ ions out of the cell and two K+ ions into the cell during every pumping cycle. Na+/K+-ATPase activity is vital for sodium and potassium homeostasis in cells, which are important for numerous enzymatic functions and secondary transport of different molecules2. Na+/K+-ATPase is a heteromeric transmembrane protein complex consisting of α- and β-subunits and a regulatory subunit belonging to the FXYD proteins. The catalytic α-subunit is responsible for transport activities and contain 10 transmembrane domains (TMDs). The β-subunit is heavily glycosylated and function as a molecular chaperone. The association of the β-subunit increases the stability of the catalytic α-subunit and dictates the affinity toward sodium and potassium. In addition, the β-subunit is necessary for the complex migration into the plasma membrane3.β-subunits has three different isoforms β-1, β-2 and β-3 that consist of small N-terminal (30-AA), a TM helix and large C-terminal (240-AA) with different tissue specificities. β-1 found to have additional role in the response of cells to oxidative stress via glutathionylation of a cysteine residue located at the center of the TMD, the same cysteine is absent in the other beta subunits4.

IHC WB

Alomone Labs

Sodium-Potassium ATPase Subunit Beta 1, ATP1B, ATP1B1

A Rabbit Polyclonal antibody to Beta 1 Na+/K+ ATPase (extracellular)

Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody (#ANP-014) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ATP1B1 from rat, mouse and human samples.

BLP-NP014

NA

Sodium-Potassium ATPase Subunit Beta 1, ATP1B, ATP1B1

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

ATP1B1

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:200

1:200-1:500