Anti-ATP1B3 (extracellular) Antibody | Alomone Labs ANP-013 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ATP1B3 (extracellular) Antibody

catalog :

ANP-013

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of human Jurkat T-cell leukemia cell line lysates (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysates (lanes 2 and 4): - 1, 2. Anti-ATP1B3 (extracellular) Antibody (#ANP-013), (1:200). 3, 4. Anti-ATP1B3 (extracellular) Antibody, preincubated with ATP1B3 (extracellular) Blocking Peptide (#BLP-NT013)

image 2 :

Expression of ATP1B3 in rat hippocampus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ATP1B3 (extracellular) Antibody (#ANP-013), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the in rat hippocampal dentate gyrus region, revealed immunoreactivity (green) in neurons of the hilus (H, arrows) and the granule layer (G). B. Pre-incubation of the antibody with ATP1B3 (extracellular) Blocking Peptide (BLP-NP013), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANP-013

SKU :

ANP-013-CF_0.2 ml

Product Name :

Anti-ATP1B3 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P54709

Applications :

IFC IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NP013

Homology :

Mouse, rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

Peptide (C)NDEVPKYRDQIPSPG, corresponding to amino acid residues 66 - 80 of human Beta 3 Na+/K+ ATPase

Product Page - Scientific background :

Na+/K+ ATPase enzyme is composed of two subunits, a large catalytic subunit (α) and a smaller glycoprotein subunit (β). The subunits assemble into a heterodimer localized to the plasma membrane1. There are three α subunits and three β subunits expressed in a tissue- and developmental-specific manner2.The Na+/K+ ATPase β subunit is composed of 302-304 amino acids, depending of the species or tissue location. The polypeptide is a single pas transmembrane protein. The protein structure is primarily helical from the amino terminus until just before the first glycosylation site3.Beta 3 Na+/K+ ATPase (ATP1B3) is highly expressed in rat lung and testis. It is also present in liver and skeletal muscle4.

Applications may also work in :

IFC IHC WB

Supplier :

Alomone Labs

Target :

Sodium/Potassium-Transporting ATPase Subunit Beta-3, CD298, Beta 3 Na+/K+ ATPase

Short Description :

A Rabbit Polyclonal Antibody to Beta 3 Na+/K+ ATPase

Long Description :

Anti-ATP1B3 (extracellular) Antibody (#ANP-013) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize ATP1B3 from rat, mouse, and human samples.

Negative Control :

BLP-NP013

Positive Control :

Synonyms :

Sodium/Potassium-Transporting ATPase Subunit Beta-3, CD298, Beta 3 Na+/K+ ATPase

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ATP1B3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage