Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody | Alomone Labs ANP-012 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody

catalog :

ANP-012

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody (#ANP-012), (1:400).2. Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody, preincubated with β 2 Na+/K+ ATPase (extracellular) Blocking Peptide (#BLP-NP012).

image 2 :

Expression of Na+/K+ ATPase β2 (ATP1B2) in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody (#ANP-012), (1:300), followed by goat anti-rabbit-AlexaFluor-488. ATP1B2 staining (green) in the hippocampal CA1 region, is detected in glial cell profiles (horizontal arrows) in the stratum radiatum (SR) and pyramidal (Pyr) layer. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANP-012

SKU :

ANP-012-CF_0.2 ml

Product Name :

Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P13638

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NP012

Homology :

Human - identical; mouse 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DTESWDQHVQKLNK, corresponding to amino acid residues 99-112 of rat ATP1B2

Product Page - Scientific background :

P-type ATPases are a large family of molecular pumps that exploit a phosphorylated enzyme intermediate in a two-step mechanism of ATP hydrolysis, cycling through states which are associated with ion transport or ion counter-transport. The Na+/K+-ATPase, a member of this family, is almost exclusively found in animals, although close homologues have been reported in certain archaea, algae and oomycetes.The Na+/K+-ATPase is comprised of a nucleotide-binding (N) and phosphorylation (P) domain, a transmembrane core (M1-M6) and a large carboxy-terminal M7-M10 segment. Na+/K+-ATPase undergoes large conformational changes as part of its functional cycle giving rise to two distinct enzymatic states: E1, which is a high-affinity state for the primary transported ion- Na+ and E2, which is the low-affinity state for the Na+ ion. The two states arise from the autocatalysed formation and breakdown of a phosphoenzyme intermediate, coupled to the binding, occlusion, translocation and release of ions. The phosphorylation site is the Asp residue of a conserved DKTGT motif. The core of the membrane transport domain encompasses transmembrane helices M1-M6, which hold the main ion-binding sites and are necessary for cytoplasmic and extracellular ion transport. A terminal R domain extension, which serves as a regulatory unit, can be found in the C-terminal of the protein. This unit is auto-inhibitory and is predicted to restrict transmembrane helix movements and/or access of ions to the membrane transport core1. Na+/K+-ATPase has been implicated in the pathogenesis of Alzheimer's Disease. A deficiency in several Na+/K+-ATPase isoform genes induce learning and memory deficits, and the α isoform is altered in Alzheimer's Disease2.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

ATP1B2, Sodium/potassium-transporting ATPase subunit beta-2, Adhesion molecule on glia, AMOG

Short Description :

A Rabbit Polyclonal Antibody to Beta 2 Na+/K+ ATPase

Long Description :

Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody (#ANP-012) is a highly specific antibody directed against an epitope of the rat ATP1B2. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope, and could potentially be used for detecting the protein in living cells. It has been designed to recognize ATP1B2 from rat, mouse, and human samples.

Negative Control :

BLP-NP012

Positive Control :

Synonyms :

ATP1B2, Sodium/potassium-transporting ATPase subunit beta-2, Adhesion molecule on glia, AMOG

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ATP1B2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage