antibody

Anti-Beta 2 Na+/K+ ATPase (extracellular)-FITC Antibody

ANP-012-F

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

ANP-012-F_50 mcl

Anti-Beta 2 Na+/K+ ATPase (extracellular)-FITC Antibody

Antibodies

Antibodies

Polyclonal

P13638

FC LCI

Human Rat Mouse

Rabbit

BLP-NP012

Human - identical, mouse 13 out of 14 amino acid residues identical

PBS pH7.4, 1% BSA with 0.05% sodium azide

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

50 µl double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

Extracellular, C-terminus

Fluorescein isothiocyanate (FITC).

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)DTESWDQHVQKLNK, corresponding to amino acid residues 99-112 of rat ATP1B2

P-type ATPases are a large family of molecular pumps that exploit a phosphorylated enzyme intermediate in a two-step mechanism of ATP hydrolysis, cycling through states which are associated with ion transport or ion counter-transport. The Na+/K+-ATPase, a member of this family, is almost exclusively found in animals, although close homologues have been reported in certain archaea, algae and oomycetes.The Na+/K+-ATPase is comprised of a nucleotide-binding (N) and phosphorylation (P) domain, a transmembrane core (M1-M6) and a large carboxy-terminal M7-M10 segment. Na+/K+-ATPase undergoes large conformational changes as part of its functional cycle giving rise to two distinct enzymatic states: E1, which is a high-affinity state for the primary transported ion- Na+ and E2, which is the low-affinity state for the Na+ ion. The two states arise from the autocatalysed formation and breakdown of a phosphoenzyme intermediate, coupled to the binding, occlusion, translocation and release of ions. The phosphorylation site is the Asp residue of a conserved DKTGT motif. The core of the membrane transport domain encompasses transmembrane helices M1-M6, which hold the main ion-binding sites and are necessary for cytoplasmic and extracellular ion transport. A terminal R domain extension, which serves as a regulatory unit, can be found in the C-terminal of the protein. This unit is auto-inhibitory and is predicted to restrict transmembrane helix movements and/or access of ions to the membrane transport core1. Na+/K+-ATPase has been implicated in the pathogenesis of Alzheimer's Disease. A deficiency in several Na+/K+-ATPase isoform genes induce learning and memory deficits, and the α isoform is altered in Alzheimer's Disease2.

FC LCI

Alomone Labs

ATP1B2, Sodium/potassium-transporting ATPase subunit beta-2, Adhesion molecule on glia, AMOG

A Rabbit Polyclonal Antibody to ?-Opioid Receptor (extracellular) conjugated to the fluorescent dye FITC

Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody (#ANP-012) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize ATP1B2 from rat, mouse and human samples. Anti-Beta 2 Na+/K+ ATPase (extracellular)-FITC Antibody (#ANP-012-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

RIC-001-F

NA

ATP1B2, Sodium/potassium-transporting ATPase subunit beta-2, Adhesion molecule on glia, AMOG

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

ATP1B2

Lyophilized powder

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis (unlabeled antibody, #ANP-012), and direct flow cytometry (labeled antibody).

1 µg peptide per 1 µg antibody

Contact Alomone for information

Contact Alomone for information