Anti-Alpha 3 Na+/K+ ATPase Antibody | Alomone Labs ANP-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Alpha 3 Na+/K+ ATPase Antibody

catalog :

ANP-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Sasahara T, Satomura K, Tada M, Kakita A, Hoshi M. Alzheimer's Aβ assembly binds sodium pump and blocks endothelial NOS activity via ROS-PKC pathway in brain vascular endothelial cells. iScience. 2021;24:102936 pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: - 1,2. Anti-Alpha 3 Na+/K+ ATPase Antibody (#ANP-003), (1:200).3,4. Anti-Alpha 3 Na+/K+ ATPase Antibody, preincubated with Alpha 3 Na+/K+ ATPase Blocking Peptide (#BLP-NP003).

image 2 :

Expression of Na+/K+ ATPase α3 (ATP1A3) in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Alpha 3 Na+/K+ ATPase Antibody (#ANP-003), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. ATP1A3 staining (green) in the hippocampal dentate gyrus is detected in the granule layer (G), (vertical arrows) and in hilar interneurons (horizontal arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANP-003

SKU :

ANP-003-CF_0.2 ml

Product Name :

Anti-Alpha 3 Na+/K+ ATPase Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P06687

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NP003

Homology :

Mouse - identical; human - 11/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DKKDDKSSPKKSK, corresponding to amino acid residues 3-15 of rat ATP1A3

Product Page - Scientific background :

Alpha 3 Na+/K+ ATPase (ATP1A3) subunit is responsible for maintaining the electrochemical gradients of Na+ and K+ across the plasma membrane. A low intracellular Na+ concentration in eukaryotic cells is maintained by the Na+/K+ ATPase protein. Na+/K+ ATPase exports three sodium ions for every two potassium ions imported1-3.In most cells, Na+/K+ ATPase is distributed along the plasma membrane, whereas in epithelia the distribution is polarized in patterns specific to the type of epithelium. In the central nervous system (CNS), the α3 Na+/K+ ATPase isoform is expressed in neurons1,3.The establishment of an electrochemical gradient for Na+ and K+ across the plasma membrane is vital for cell function, it determines cell resting membrane potentials and supports the electrical activity of excitable cells. Autosomal dominant mutations of the human gene are related to a wide spectrum of clinical conditions, including several defined syndromes such as rapid-onset dystonia-parkinsonism, hyperactivity, epileptic seizures, optic atrophy, and sensorineural hearing loss2,3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

ATP1A3, Sodium/potassium ATPase catalytic subunit alpha-3, Sodium pump subunit alpha-3, Na+/K+-transporting ATPase subunit alpha-3

Short Description :

A Rabbit Polyclonal Antibody to Alpha 3 Na+/K+ ATPase

Long Description :

Anti-Alpha 3 Na+/K+ ATPase Antibody (#ANP-003) is a highly specific antibody directed against an epitope of the rat ATP1A3. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ATP1A3 from rat, mouse, and human samples.

Negative Control :

BLP-NP003

Positive Control :

Synonyms :

ATP1A3, Sodium/potassium ATPase catalytic subunit alpha-3, Sodium pump subunit alpha-3, Na+/K+-transporting ATPase subunit alpha-3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ATP1A3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage