Anti-Alpha 2 Na+/K+ ATPase Antibody | Alomone Labs ANP-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Alpha 2 Na+/K+ ATPase Antibody

catalog :

ANP-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-Alpha 2 Na+/K+ ATPase Antibody (#ANP-002), (1:200).3,4. Anti-Alpha 2 Na+/K+ ATPase Antibody, preincubated with Alpha 2 Na+/K+ ATPase Blocking Peptide (#BLP-NP002).

image 2 :

Expression of Na+/K+ ATPase α2 (ATP1A2) in mouse hippocampus and cerebellum - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Alpha 2 Na+/K+ ATPase Antibody (#ANP-002), (1:300), followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody. A. ATP1A2 staining (green) in mouse hippocampal CA3 region is labeled in astrocytic processes (arrows surrounding pyramidal cells, P) and diffused staining is observed in the CA3 stratum radiatum. Note absence of staining in the mossy fiber terminal region (MF). B. Staining in mouse cerebellum shows labeling of the basket shape (arrows) under Purkinje cells (P) and diffused staining in the molecular layer (MOL). Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANP-002

SKU :

ANP-002-CF_0.2 ml

Product Name :

Anti-Alpha 2 Na+/K+ ATPase Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P06686

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NP002

Homology :

Mouse, human, dog, pig - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)REYSPAATTAENG, corresponding to amino acid residues 7-19 of rat ATP1A2

Product Page - Scientific background :

The Na+/K+ pumps are members of the P-type family of active cation transport proteins. They consume ATP to actively transport Na+ out of and K+ into the cell. Na+ and K+ gradients are required for maintaining membrane potential, cell volume and secondary transport of other solutes thus making these pumps essential for all mammalian cells. The minimum functional unit of the Na+/K+ pump consists of a heterodimer of α- and β- subunits1. The α subunit (also known as ATP1A) has a molecular mass of about 100 kDa and four distinct isoforms have been identified so far. It has ten transmembrane segments, short extracellular loops and larger cytoplasmic regions. There is a large central loop between M4 and M5 composed of 430 amino acid residues and a long N-terminal tail of about 90 amino acid residues. The nucleotide-binding domain (N domain) and phosphorylation domain (P domain) of the pump are formed by the M4/M5 loop while the actuator domain (A domain) is formed by the amino-terminal tail and the M2/M3 loop. The major isoform, α1, is found in most tissues and is the major form in the kidney and in most epithelia cells. The β-subunit is essential for the delivery and insertion of the α-subunit into the cell's membrane, whilst β-subunit delivery to the membrane is not dependent on α-subunit expression2. ATP1A2 mutations that cause diverse functional abnormalities of the Na+/K+ pump have been implicated in the pathogenesis of familial hemiplegic migraine3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

ATP1A2, Sodium/potassium ATPase catalytic subunit alpha-2, Sodium pump subunit alpha-2

Short Description :

A Rabbit Polyclonal Antibody to Alpha 2 Na+/K+ ATPase

Long Description :

Anti-Alpha 2 Na+/K+ ATPase Antibody (#ANP-002) is a highly specific antibody directed against an epitope of the rat ATP1A2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ATP1A2 from human, rat, and mouse samples.

Negative Control :

BLP-NP002

Positive Control :

Synonyms :

ATP1A2, Sodium/potassium ATPase catalytic subunit alpha-2, Sodium pump subunit alpha-2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ATP1A2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage