Anti-Alpha 1 Na+/K+ ATPase Antibody | Alomone Labs ANP-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Alpha 1 Na+/K+ ATPase Antibody

catalog :

ANP-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:1000; loading ...; fig 2h	Liu X, Ge J, Chen C, Shen Y, Xie J, Zhu X, et al. FAT10 protects against ischemia-induced ventricular arrhythmia by decreasing Nedd4-2/Nav1.5 complex formation. Cell Death Dis. 2021;12:25 pubmed publisher
	Yoon S, Bogdanov K, Wallach D. Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes. Cell Death Differ. 2022;: pubmed publisher
	Nof E, Vysochek L, Meisel E, Burashnikov E, Antzelevitch C, Clatot J, et al. Mutations in NaV1.5 Reveal Calcium-Calmodulin Regulation of Sodium Channel. Front Physiol. 2019;10:700 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001), (1:200).3,4. Anti-Alpha 1 Na+/K+ ATPase Antibody, preincubated with Alpha 1 Na+/K+ ATPase Blocking Peptide (#BLP-NP001).

image 2 :

Expression of Na+/K+ ATPase α1 (ATP1A1) in rat kidney - Immunohistochemical staining of paraffin embedded rat kidney sections using Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001), (1:200). ATP1A1 staining (brown) is present in collecting ducts and thick portions of the Henle loop in the renal medulla. Hematoxilin is used as the counterstain.

image 3 :

Peptide (C)RDKYEPAAVSEHGD corresponding to amino acid residues 7- 20 of rat ATP1A1(AccessionP06685). Intracellular N-terminus.

product information

CAT :

ANP-001

SKU :

ANP-001-CF_0.2 ml

Product Name :

Anti-Alpha 1 Na+/K+ ATPase Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P06685

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NP001

Homology :

Mouse, dog, pig - identical; Human- 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RDKYEPAAVSEHGD, corresponding to amino acid residues 7- 20 of rat ATP1A1

Product Page - Scientific background :

Na+/K+-ATPase, a membrane protein located on the plasma membrane of all animal cells, is involved in maintaining the Na+ and K+ gradients across the cell membrane1. It is ubiquitously expressed in neurons and helps maintain normal neuronal function2. The enzyme is composed of a catalytic α chain of 112 kDa. and α glycosylated β chain of 40-50 kDa. There are four isoforms of the α subunit (α1, α2, α3 and α4) and three isoforms of the β subunit (β1, β2 and β3) with different tissue distributions3. The N-terminus is located on the cytoplasmic side of the membrane. There are four transmembrane segments in the first third of the polypeptide chain, a large cytoplasmic loop (LCL) comprising the second third of the chain and four to six transmembrane segments in the C-terminal third of the protein with the C-terminus also on the cytoplasmic side of the membrane4. Na+/K+-ATPase abnormalities have been reported to be involved in several neurological diseases such as seizures, bipolar disorder, spongiform encephalopathy, and Alzheimer's disease. Na+/K+-ATPase may also have implications in behavioral defects5.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

ATP1A1, Sodium/potassium ATPase catalytic subunit alpha-1, Sodium pump subunit alpha-1

Short Description :

A Rabbit Polyclonal Antibody to Alpha 1 Na+/K+ ATPase

Long Description :

Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001) is a highly specific antibody directed against an epitope of the rat ATP1A1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ATP1A1 from rat, mouse, and human samples.

Negative Control :

BLP-NP001

Positive Control :

Synonyms :

ATP1A1, Sodium/potassium ATPase catalytic subunit alpha-1, Sodium pump subunit alpha-1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ATP1A1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200-1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage