antibody

Guinea Pig Anti-Alpha 1 Na+/K+ ATPase Antibody

ANP-001-GP

polyclonal

guinea-pigs

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry

ANP-001-GP-CF_0.2 ml

Guinea Pig Anti-Alpha 1 Na+/K+ ATPase Antibody

Antibodies

Antibodies

Polyclonal

P06685

IHC WB

Human Rat Mouse

Guinea Pig

BLP-NP001

Mouse, dog, pig - identical, Human- 13 out of 14 amino acid residues identical

PBS pH7.4

Guinea pig total IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Intracellular, N-terminus

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)RDKYEPAAVSEHGD, corresponding to amino acid residues 7- 20 of rat ATP1A1

Na+/K+-ATPase, a membrane protein located on the plasma membrane of all animal cells, is involved in maintaining the Na+ and K+ gradients across the cell membrane1. It is ubiquitously expressed in neurons and helps maintain normal neuronal function2. The enzyme is composed of a catalytic α chain of 112 kDa. and α glycosylated β chain of 40-50 kDa. There are four isoforms of the α subunit (α1, α2, α3 and α4) and three isoforms of the β subunit (β1, β2 and β3) with different tissue distributions3. The N-terminus is located on the cytoplasmic side of the membrane. There are four transmembrane segments in the first third of the polypeptide chain, a large cytoplasmic loop (LCL) comprising the second third of the chain and four to six transmembrane segments in the C-terminal third of the protein with the C-terminus also on the cytoplasmic side of the membrane4. Na+/K+-ATPase abnormalities have been reported to be involved in several neurological diseases such as seizures, bipolar disorder, spongiform encephalopathy, and Alzheimer's disease. Na+/K+-ATPase may also have implications in behavioral defects5.

IHC WB

Alomone Labs

ATP1A1, Sodium/potassium ATPase catalytic subunit alpha-1, Sodium pump subunit alpha-1

A Guinea Pig Polyclonal Antibody to Alpha 1 Na+/K+ ATPase

Guinea Pig Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001-GP) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ATP1A1 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

BLP-NP001

NA

ATP1A1, Sodium/potassium ATPase catalytic subunit alpha-1, Sodium pump subunit alpha-1

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

ATP1A1

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:300

1:400