Anti-Lynx1 (extracellular) Antibody | Alomone Labs ANC-021 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Lynx1 (extracellular) Antibody

catalog :

ANC-021

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1, 2. Anti-Lynx1 (extracellular) Antibody (#ANC-021), (1:200). 3, 4. Anti-Lynx1 (extracellular) Antibody, preincubated with Lynx1 (extracellular) Blocking Peptide (#BLP-NC021).

image 2 :

Expression of Lynx1 in rat dorsal raphe nucleus and substantia nigra pars compacta - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Lynx1 (extracellular) Antibody (#ANC-021), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. Lynx1 staining in the dorsal raphe nucleus (DRN), (green) is detected in cells of the medial DRN (MDRN, horizontal arrows) and in cells of the lateral DRN (LDRN), (vertical arrows). B. Lynx1 immunoreactivity in the substantia nigra pars compacta (SNC), (green) is observed in cells of the SNC (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANC-021

SKU :

ANC-021-CF_0.2 ml

Product Name :

Anti-Lynx1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P0DP60

Applications :

IF IFC IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NC021

Homology :

Mouse, rat - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

CAYNGDN(S)FKPMR, corresponding to amino acid residues 26-38 of mouse Lynx1

Product Page - Scientific background :

Lynx1 is a member of the Ly-6/neurotoxin gene superfamily (Lynx family) of proteins that feature a three-looped toxin fold and a cysteine rich motif; structural characteristics that are shared with the snake venom toxin α-bungarotoxin1.Lynx1 is expressed in large projection neurons in the hippocampus, cortex, and cerebellum. Initial analysis of the functional consequence of genetic ablation of Lynx1 in mice revealed that Lynx1 is an allosteric modulator of various nicotinic receptors that affects their kinetics, decreases their agonist affinities, and increases their desensitization to overall limit their activation. Lynx1 can serve as a protective measure against neurotoxicity through agonist-induced overstimulation2. In fact, deletion of Lynx1 eventually leads to neurodegeneration in aged mice3.Collectively the findings regarding Lynx1 suggest a direct relationship between plasticity regulation and nicotinic signaling, and underline the significance of fine tuning of nicotinic tone to vastly control the development of brain functions.Lynx1 activity was shown to be related to the pathophysiology of Alzheimer's disease as well as cholinergic activity in healthy and tumor cells of bronchial epithelium4.

Applications may also work in :

IF IFC IHC WB

Supplier :

Alomone Labs

Target :

Ly-6/Neurotoxin-like protein 1, Endogenous prototoxin LYNX1

Short Description :

A Rabbit Polyclonal Antibody to Lynx1

Long Description :

Anti-Lynx1 (extracellular) Antibody (#ANC-021) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, indirect live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize Lynx1 from rat, mouse, and human samples.

Negative Control :

BLP-NC021

Positive Control :

Synonyms :

Ly-6/Neurotoxin-like protein 1, Endogenous prototoxin LYNX1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

LYNX1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1000

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage