Anti-RIC3 Antibody | Alomone Labs ANC-020 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-RIC3 Antibody

catalog :

ANC-020

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Deshpande A, Vinayakamoorthy R, Garg B, Thummapudi J, Oza G, Adhikari K, et al. Why Does Knocking Out NACHO, But Not RIC3, Completely Block Expression of α7 Nicotinic Receptors in Mouse Brain?. Biomolecules. 2020;10: pubmed publisher

image

image 1 :

Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate: - 1. Anti-RIC3 Antibody (#ANC-020), (1:200).2. Anti-RIC3 Antibody, preincubated with RIC3 Blocking Peptide (#BLP-NC020).

image 2 :

Expression of RIC-3 in mouse olfactory bulb and fornix - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-RIC3 Antibody (#ANC-020), (1:400), followed by goat-anti-rabbit-AlexaFluor-488. A. RIC-3 staining in mouse olfactory bulb shows staining in the external plexiform layer (EPL) and in some glomeruli (G). Arrows point at glomeruli with RIC-3 positive core. B. RIC-3 staining in mouse fornix is detected in neuronal profiles (vertical arrows) and neuronal processes (horizontal arrow). Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANC-020

SKU :

ANC-020-CF_0.2 ml

Product Name :

Anti-RIC3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8BPM6

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NC020

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)EKLINRVGPNGESR, corresponding to amino acid residues 161-174 of mouse RIC3

Product Page - Scientific background :

RIC-3 (Resistance to inhibitors of cholinesterase 3) is a conserved endoplasmic reticulum-resident protein that is known to be a chaperone for the nicotinic acetylcholine receptors (nAChR) and for the serotonin (5-HT3) channels1.RIC-3 affects the maturation of multiple nAChRs which has been shown to facilitate nAChR assembly and trafficking3,4.Multiple transcripts of RIC-3 displaying differential expression are detected in humans. The full-length transcript includes two transmembrane domains followed by a coiled-coil domain, a nonconserved N-terminus and C-terminus domain, which includes a second coiled-coil domain. The short isoform lacks the coiled-coil domains and is very conserved in mammals1,2,4.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Protein RIC-3, RIC3 acetylcholine receptor chaperone, Resistant to inhibitor of cholinesterase 3

Short Description :

A Rabbit Polyclonal Antibody to RIC-3

Long Description :

Anti-RIC3 Antibody (#ANC-020) is a highly selective antibody directed against an epitope of mouse protein RIC-3. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize RIC3 from human, mouse, and rat samples.

Negative Control :

BLP-NC020

Positive Control :

Synonyms :

Protein RIC-3, RIC3 acetylcholine receptor chaperone, Resistant to inhibitor of cholinesterase 3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

RIC3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage