Anti-Nicotinic Acetylcholine Receptor α9 (CHRNA9) Antibody | Alomone Labs ANC-019 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Nicotinic Acetylcholine Receptor α9 (CHRNA9) Antibody

catalog :

ANC-019

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2. Anti-Nicotinic Acetylcholine Receptor α9 (CHRNA9) Antibody (#ANC-019), (1:400).3,4. Anti-Nicotinic Acetylcholine Receptor α9 (CHRNA9) Antibody, preincubated with Nicotinic Acetylcholine Receptor α9/CHRNA9 Blocking Peptide (#BLP-NC019).

image 2 :

Expression of nAChRα9 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Nicotinic Acetylcholine Receptor α9 (CHRNA9) Antibody (#ANC-019), (1:400), followed by goat-anti-rabbit-AlexaFluor-488. nAChRα9 staining (in green) appears in neuronal soma (arrows). DAPI is used as the counterstain (blue).

product information

CAT :

ANC-019

SKU :

ANC-019-CF_0.2 ml

Product Name :

Anti-Nicotinic Acetylcholine Receptor α9 (CHRNA9) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P43144

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NC019

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KDHKATNSKGSEWKK, corresponding to amino acid residues 436-450 of rat nAChRα9

Product Page - Scientific background :

The nicotinic acetylcholine receptors (nAChRs) are ionotropic multi-subunit, neurotransmitter-gated receptors of the cholinergic system. These receptors are responsible for mediating the effects of the neurotransmitter acetylcholine (ACh). They are assembled from one or more α subunits (α1-α10) alone or together with one or more β subunits (β1-β4).The receptors are also a target of the biologic compound nicotine, which mostly mimics the effects of acetylcholine on the receptors by binding as an agonist to α subunit of nAChRs.nAChRs play critical physiologic roles in the central and peripheral nervous systems. They regulate neurotransmitter release, cell excitability, neuronal integration, and are involved in functions such as sleep and arousal patterns, fatigue, hunger, anxiety, and pain processing1,2.The structure of each subunit is composed of an extracellular domain, which harbors the binding site, four transmembrane α-helices (TM1-4), and a variable intracellular region4. The α subunits have a defining "cysteine loop" that contains two vicinal cysteine residues3.nAChRs containing the α9 subunit are expressed in a variety of non-neuronal tissues starting from immune cells to breast carcinomas. The α9 subunit is able to form a functional homomeric receptor and in addition to co-assemble with the α10 subunit into functional heteromeric nAChRs3.α9-containing nAChRs play an important role in pain, inflammation, keratinocyte adhesion and in mediating synaptic transmission between the efferent olivocochlear fibers and cochlear hair cells3.nAChRs are involved in pathologies, such as myasthenia, epilepsy, schizophrenia, Parkinson's disease, autism, dementia with Lewy bodies, Alzheimer's disease, and addiction3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

nAChR α9, nAChR alpha-9, Neuronal acetylcholine receptor subunit alpha-9, ACRA9

Short Description :

A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α9

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat nAChRα9. Anti-Nicotinic Acetylcholine Receptor α9 (CHRNA9) Antibody (#ANC-019) can be used western blot and immunohistochemistry applications. It has been designed to recognize nAChRα9 from human, rat and mouse samples.

Negative Control :

BLP-NC019

Positive Control :

Synonyms :

nAChR α9, nAChR alpha-9, Neuronal acetylcholine receptor subunit alpha-9, ACRA9

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CHRNA9

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage