Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular)-ATTO Fluor-594 Antibody | Alomone Labs ANC-014-AR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular)-ATTO Fluor-594 Antibody

catalog :

ANC-014-AR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 594

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Expression of nAChR β4 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular)-ATTO Fluor-594 Antibody (#ANC-014-AR), (1:100). A. nAChRβ4 labeling (red) appears in neuronal cell bodies. B. Nuclear staining using DAPI as counterstain (blue). C. Merged images of A and B.

image 2 :

Expression of nAChR ?4 in human SH-SY5Y cells - Cell surface detection ofnAChR ?4 in live intact human SH-SY5Y neuroblastoma cells. A. Cells were stained withAnti-Nicotinic Acetylcholine Receptor ?4 (CHRNB4) (extracellular)-ATTO-594 Antibody(#ANC-014-AR) (1:100) (red). B. Live image of the cells. C. Merge of the two images.

image 3 :

Cell surface detection of PLXNA3 in live intact human THP-1 monocytic leukemia cells: - ___Cells.___Cells + goat-anti-rabbit-FITC.___Cells +Anti-Plexin-A3 (extracellular) Antibody (#APR-093) (2.5 g) + goat-anti-rabbit-FITC.

product information

CAT :

ANC-014-AR

SKU :

ANC-014-AR_50 mcl

Product Name :

Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular)-ATTO Fluor-594 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P12392

Applications :

IC IF IHC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NC014

Homology :

Mouse - identical; human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CYEGVNILRIPAKR, corresponding to amino acid residues 95-108 of rat nAChRβ4

Product Page - Scientific background :

Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs), which belong to the G-protein coupled receptor (GPCR) superfamily, and nicotinic AChRs (nAChRs), which belong to the ligand-gated ion channel superfamily. nAChRs also respond to nicotine, hence their name1.To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of α subunits (α1-10), which are responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding2. The other subunits responsible for making up the active receptor are the β (β1-4), γ, δ and ε subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, three conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer of these various subunits organized around a central pore1.While most β subunits are neuronal, the β1 subunit forms functional receptors along with other subunits in the muscle3. In neurons the α2-α6 and β2-β4 subunits form heteropentameric receptors, usually with a (αx)2(βy)3 stoichiometry3.

Applications may also work in :

IC IF IHC LCI

Supplier :

Alomone Labs

Target :

nAChR β4, Neuronal acetylcholine receptor subunit beta-4, Cholinergic receptor nicotinic beta 4

Short Description :

A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β4 Conjugated to the Fluorescent Dye ATTO-594

Long Description :

Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody (#ANC-014) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It and has been designed to recognize nAChRβ4 from mouse, rat, and human samples. Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular)-ATTO Fluor-594 Antibody (#ANC-014-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular)-ATTO Fluor-594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

BLP-NC014

Positive Control :

Synonyms :

nAChR β4, Neuronal acetylcholine receptor subunit beta-4, Cholinergic receptor nicotinic beta 4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CHRNB4

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ANC-014), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage