Anti-Nicotinic Acetylcholine Receptor β3 (CHRNB3) (extracellular) Antibody

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Nicotinic Acetylcholine Receptor β3 (CHRNB3) (extracellular) Antibody

catalog :

ANC-013

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-Nicotinic Acetylcholine Receptor β3 (CHRNB3) (extracellular) Antibody (#ANC-013), (1:400).2. Anti-Nicotinic Acetylcholine Receptor β3 (CHRNB3) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor β3/CHRNB3 (extracellular) Blocking Peptide (#BLP-NC013).

image 2 :

Expression of nAChR ?3 in live intact rat PC12 cells - Cell surface detection ofnAChR ?3 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells withAnti-Nicotinic Acetylcholine Receptor ?3 (CHRNB3) (extracellular) Antibody(#ANC-013) (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge image of A and B.

product information

CAT :

ANC-013

SKU :

ANC-013-CF_0.2 ml

Product Name :

Anti-Nicotinic Acetylcholine Receptor β3 (CHRNB3) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P12391

Applications :

IC IF LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NC013

Homology :

Mouse - identical; human - 11/12 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)NAKGMKGNRREG, corresponding to amino acid residues 211-222 of rat nAChRβ3

Product Page - Scientific background :

Nicotinic acetylcholine receptors (nAChRs) mediate the physiological effects of exogenous nicotine. They also play critical physiological roles throughout the brain and body by mediating cholinergic excitatory neurotransmission, modulating the release of neurotransmitters, and having longer-term effects on gene expression and cellular connections1.nAChRs are pentameric complexes made up of combinations of a number of different nAChR subunits. To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of α subunits (α1-10), which is responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding2. The other subunits responsible for making up the active receptor are the β (β1-4), γ, δ and ε subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, three conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer of these various subunits organized around a central pore1. The β3 subunit cannot form functional receptors when expressed alone. It only forms operational channels when coexpressed with other functional subunit combinations, and is thus commonly termed 'orphan' or 'auxiliary' subunits3.β3 nAChR subunit mRNA is highly expressed in dopaminergic neurons of the substantia nigra and ventral tegmental area4.α6/β3 nAChRs have been implicated in dopaminergic neurotransmission, nicotine dependence, anxiety, and other important neurophysiological processes5.

Applications may also work in :

IC IF LCI WB

Supplier :

Alomone Labs

Target :

nAChR β3, Neuronal acetylcholine receptor subunit beta-3

Short Description :

A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β3

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat nAChR β3. Anti-Nicotinic Acetylcholine Receptor β3 (CHRNB3) (extracellular) Antibody (#ANC-013) can be used in western blot and live cell imaging applications. It has been designed to recognize nAChRβ3 from mouse, rat and human samples.

Negative Control :

BLP-NC013

Positive Control :

Synonyms :

nAChR β3, Neuronal acetylcholine receptor subunit beta-3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CHRNB3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage