antibody

Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody

ANC-012

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry, immunocytochemistry

ANC-012-CF_0.2 ml

Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

P12390

IC IF IHC LCI WB

Human Rat Mouse

Rabbit

BLP-NC012

Mouse - identical; human - 11/12 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Extracellular, N-terminus

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)EDFDNMKKVRLP, corresponding to amino acid residues 96-107 of rat nAChRβ2

Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs), which belong to the G-protein coupled receptor (GPCR) superfamily, and nicotinic AChRs (nAChRs), which belong to the ligand-gated ion channel superfamily. nAChRs also respond to nicotine, hence their name1.To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of α subunits (α1-10), which is responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding2. The other subunits responsible for making up the active receptor are the β (β1-4), γ, δ and ε subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, three conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer of these various subunits organized around a central pore1.While most β subunits are neuronal, the β1 subunit forms functional receptors along with other subunits in the muscle3. The diversity of these receptors and their functional organization gives rise to unique properties and functions. The α4β2 receptor composition makes up a high affinity nicotinic receptor. In fact, its upregulation (mainly expressed by the increase of functional receptors at the membrane and not expression per se) is responsible for the increased appearance of binding sites following nicotine administration1,3.Animal studies have shown that nAChR-related mechanisms are involved in attention function4. Indeed α4β2 nAChR seems to also be involved in attention-deficit hyperactivity disorder (ADHD), a disease distinguished by a lack of attention, distractibility and hyperactivity3. The α4β2 and α7 nAChRs appear to be critical in rats for attention and working memory. Also, a α4β2 specific agonist was shown to reduce impulsivity, hyperactivity and attention deficits in adults with ADHD5. This same receptor subtype may also be involved in Parkinson's disease (PD) as smoking and α4β2 nAChR agonists show beneficial effects in PD6,7.

IC IF IHC LCI WB

Alomone Labs

nAChR β2, Neuronal acetylcholine receptor subunit beta-2, Cholinergic receptor nicotinic beta 2, ACRB2, EFNL3

A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β2

Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody (#ANC-012) is a highly specific antibody directed against an epitope the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize nAChRβ2 from mouse, rat, and human samples.

BLP-NC012

NA

nAChR β2, Neuronal acetylcholine receptor subunit beta-2, Cholinergic receptor nicotinic beta 2, ACRB2, EFNL3

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

CHRNB2

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

IHC

NA

Western blot analysis

1 µg peptide per 1 µg antibody

Contact Alomone for information

1:200