Anti-Nicotinic Acetylcholine Receptor β1 (CHRNB1) (extracellular) Antibody

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Nicotinic Acetylcholine Receptor β1 (CHRNB1) (extracellular) Antibody

catalog :

ANC-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) heart lysates: - 1,2. Anti-Nicotinic Acetylcholine Receptor β1 (CHRNB1) (extracellular) Antibody (#ANC-011), (1:500).3,4. Anti-Nicotinic Acetylcholine Receptor β1 (CHRNB1) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor β1/CHRNB1 (extracellular) Blocking Peptide (#BLP-NC011).

image 2 :

Peptide (C)EKDEEMSTKVYLD corresponding to amino acid residues 68-80 of rat nAChR?1 (AccessionP25109). Extracellular N-terminus.

image 3 :

Alomone Labs Sitaxsentan sodium salt inhibits ETA receptor-mediated Ca2+ mobilization in CHO cells. - Dose response curve ofSitaxsentan sodium salt(#S-185) inhibition of the ET-A receptor-mediated Endothelin-1-evoked Ca2+mobilization. IC50was determined at 9.29 nM. Cells were loaded with Calcium 6 dye incubated with Sitaxsentan sodium salt and stimulated with 15 nM Endothelin-1 (EC80). Changes in intracellular Ca2+levels were detected as changes in maximum relative fluorescence (RLU) using FLIPRTETRA?.

product information

CAT :

ANC-011

SKU :

ANC-011-CF_0.2 ml

Product Name :

Anti-Nicotinic Acetylcholine Receptor β1 (CHRNB1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P25109

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NC011

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EKDEEMSTKVYLD, corresponding to amino acid residues 68-80 of rat nAChRβ1

Product Page - Scientific background :

Neuronal nicotinic acetylcholine receptors (nAChRs) belong to the superfamily of ligand-gated ion channels and are widely expressed throughout the central and peripheral nervous systems. nAChRs play crucial roles in modulating a wide range of higher cognitive functions by mediating presynaptic, postsynaptic, and extrasynaptic signaling1.nAChRs are formed by the assembly of five transmembrane subunits, selected from a pool of 17 homologous polypeptides (α1-10, β1-4, γ, δ, and ε). There are many nAChR subtypes, each consisting of a specific combination of subunits, which mediate diverse physiological functions. They are widely expressed in the central nervous system, while, in the periphery, they mediate synaptic transmission at the neuromuscular junction and ganglia. nAChRs are also found in non-neuronal/non-muscle cells (keratinocytes, epithelia, macrophages, etc.)2. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, three conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer of these various subunits organized around a central pore3.While most β subunits are neuronal, the β1 subunit forms functional receptors along with other subunits in the muscle2. β1 subunit seems to be involved in myasthenia gravis (MG)4, an acquired autoimmune disease usually characterized by the presence of circulating autoantibodies that bind to and destroy muscle nAChRs5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

nAChR β1, ACHRB

Short Description :

A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat nAChR β1. Anti-Nicotinic Acetylcholine Receptor β1 (CHRNB1) (extracellular) Antibody (#ANC-011) can be used in western blot analysis. As the antibody recognizes an extracellular epitope, it can be used to detect the receptor in living cells. It has been designed to nAChRβ1 from rat, mouse and human samples.

Negative Control :

BLP-NC011

Positive Control :

Synonyms :

nAChR β1, ACHRB

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CHRNB1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage