antibody

Anti-Nicotinic Acetylcholine Receptor α10 (CHRNA10) Antibody

ANC-010

polyclonal

domestic rabbit

nonconjugated

NA

mouse, rat

ANC-010-CF_0.2 ml

Anti-Nicotinic Acetylcholine Receptor α10 (CHRNA10) Antibody

Antibodies

Antibodies

Polyclonal

Q9JLB5

WB

Rat Mouse

Rabbit

BLP-NC010

Mouse - identical; human - 13/15 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

2nd intracellular loop

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)RRHEDWKRLARVMDR, corresponding to amino acid residues 411-425 of rat nAChRα10

Ligand-gated ion channels are expressed throughout the brain where they mediate fast synaptic transmission. The nicotinic acetylcholine receptors (nAChRs) represent an important subcategory of these ligand-gated ion channels; nAChRs are multisubunit, neurotransmitter-gated receptors of the cholinergic system.All nicotinic acetylcholine receptors are pentamers composed of homologous protein subunits, each subunit spanning the plasma membrane four times. There are 10 α-subunits (α1-α10), 4 β-subunits (β1-β4), and γ-, δ-, and ϵ-subunits, all with shared homology. Various combinations of subunits form subtypes of the acetylcholine receptor. α-subunits of CHRNA10 consist of a N-terminal extracellular domain that contains the ACh binding region. There are four hydrophilic membrane-spanning domains (often labeled as M1-4) and an intracellular loop between M3 and M4.The α10 gene encoding the CHRNA10 subunit is mostly expressed in outer hair cells in the ear. To this day, the α10 receptor has only been shown to form a functional receptor when combined with α9. As compared to expressed homomeric α9 receptors, the heteromeric α9α10 receptors display faster and more extensive agonist-mediated desensitization, a distinct current-voltage relationship, and a biphasic response to changes in extracellular Ca2+ ions. The primary role identified for CHRNA10 function within the inner ear is related to efferent auditory neuronal activity1.CHRNA10 knockout does not cause major alterations either in the functional expression of other voltage-gated and/or Ca2+-sensitive K+ channels or in the electrical properties of the cochlear inner hearing cells2.

WB

Alomone Labs

nAChR α10, Cholinergic receptor nicotinic alpha 10, Neuronal acetylcholine receptor subunit alpha-10

A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α10

Anti-Nicotinic Acetylcholine Receptor α10 (CHRNA10) Antibody (#ANC-010) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize nAChRα10 from rat, mouse, and human samples.

BLP-NC010

NA

nAChR α10, Cholinergic receptor nicotinic alpha 10, Neuronal acetylcholine receptor subunit alpha-10

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

CHRNA10

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

Contact Alomone for information

1:400-1:500