Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody

catalog :

ANC-006

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Cardenas A, Elabd M, Lotfipour S. Specificity of a rodent alpha(α)6 nicotinic acetylcholine receptor subunit antibody. Psychopharmacology (Berl). 2019;: pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2. Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody (#ANC-006), (1:1000).3,4. Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α6/CHRNA6 (extracellular) Blocking Peptide (#BLP-NC006).

image 2 :

Expression of nAChRα6 in rat striatum - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody (#ANC-006), (1:100). A. nAChRα6 staining (red) is detected in the striatal matrix (M) but not in the striatal patches (arrows) nor in the overlying corpus callosum (CC). B. DAPI (blue) counterstain reveals the outline of the striatal matrix and patches and the overlying corpus callosum.

image 3 :

Expression of nAChR?6 in rat PC12 cells - Cell surface detection ofnAChR?6 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells withAnti-Nicotinic Acetylcholine Receptor ?6 (CHRNA6) (extracellular) Antibody(#ANC-006) (1:50) followed by goat anti-rabbit-AlexaFluor- 594secondary antibody (red). B. Live image of the cells. C. Merge of the two images.

product information

CAT :

ANC-006

SKU :

ANC-006-CF_0.2 ml

Product Name :

Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P43143

Applications :

IC IF IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NC006

Homology :

Mouse - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EQLFHTLFAHYNR, corresponding to amino acid residues 35-47 of rat nAChRα6

Product Page - Scientific background :

Neuronal nicotinic acetylcholine receptors (nAChRs) belong to the superfamily of ligand-gated ion channels and are widely expressed throughout the central and peripheral nervous systems. nAChRs play crucial roles in modulating a wide range of higher cognitive functions by mediating presynaptic, postsynaptic, and extrasynaptic signaling1.Nicotinic acetylcholine receptors containing α6 subunits (nAChRα6) exhibit a unique expression pattern in the mammalian brain. nAChRs α6 are abundantly expressed in the midbrain dopaminergic (DAergic) system, including mesocorticolimbic and nigrostriatal pathways, and particularly present in presynaptic nerve terminals2. nAChRα6 expressed on DAergic neurons can be activated by endogenous acetylcholine (Ach) or exogenous nicotine and analogs, which suggests that the activation of nAChRα6 may play a vital role in central cholinergic circuits, including modulation of locomotor behaviour and drug addiction3. In addition, nAChRα6 is particularly susceptible to nigrostriatal damage, which may lead to Parkinson's disease (PD)4. nAChRα6 are also expressed by sensory neurons; therefore, they may also play a role in disorders related to sensory perception5.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

nAChR α6, Cholinergic receptor nicotinic alpha 6, Neuronal acetylcholine receptor subunit alpha-6, ACRA6

Short Description :

A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α6

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat nAChRα6. Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody (#ANC-006) can be used in western blot, live cell imaging (immunocytochemistry) and immunohistochemistry applications. It has been designed to recognize nAChRα6 from rat and mouse samples. The antibody is not recommended for human samples.

Negative Control :

BLP-NC006

Positive Control :

Synonyms :

nAChR α6, Cholinergic receptor nicotinic alpha 6, Neuronal acetylcholine receptor subunit alpha-6, ACRA6

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CHRNA6

AB Specifictiy :

Not recommended for human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:1000

more info or order :

Alomone Labs product webpage