Anti-Nicotinic Acetylcholine Receptor α5 (CHRNA5) (extracellular) Antibody

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Nicotinic Acetylcholine Receptor α5 (CHRNA5) (extracellular) Antibody

catalog :

ANC-005

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Ganic E, Singh T, Luan C, Fadista J, Johansson J, Cyphert H, et al. MafA-Controlled Nicotinic Receptor Expression Is Essential for Insulin Secretion and Is Impaired in Patients with Type 2 Diabetes. Cell Rep. 2016;14:1991-2002 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) enriched brain membranes: - 1,2. Anti-Nicotinic Acetylcholine Receptor α5 (CHRNA5) (extracellular) Antibody (#ANC-005), (1:200).3,4. Anti-Nicotinic Acetylcholine Receptor α5 (CHRNA5) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α5/CHRNA5 (extracellular) Blocking Peptide (#BLP-NC005).

image 2 :

Peptide (C)EGASTKTVVRYNGT corresponding to amino acid residues 129-142 of rat nAChR?5 (AccessionP20420). Extracellular N-terminus.

image 3 :

Alomone Labs Sitaxsentan inhibits ET-A receptor-mediated Ca2+mobilization in CHO cells. - Dose response plot ofSitaxsentan(#S-186) inhibition of the ET-A receptor-mediated Endothelin-1-evoked Ca2+mobilization. IC50was determined at 11.67 nM. Cells were loaded with Calcium 6 dye incubated with Sitaxsentan and stimulated with 15 nM Endothelin-1 (EC80). Changes in intracellular Ca2+levels were detected aschanges in maximum relative fluorescence (RLU) using FLIPRTETRA?.

product information

CAT :

ANC-005

SKU :

ANC-005-CF_0.2 ml

Product Name :

Anti-Nicotinic Acetylcholine Receptor α5 (CHRNA5) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P20420

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NC005

Homology :

Mouse - identical; human - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EGASTKTVVRYNGT, corresponding to amino acid residues 129-142 of rat nAChRα5

Product Page - Scientific background :

Nicotinic acetylcholine receptors (nAChRs) mediate the physiological effects of exogenous nicotine. They also play critical physiological roles throughout the brain and body by mediating cholinergic excitatory neurotransmission, modulating the release of neurotransmitters, and having longer-term effects on gene expression and cellular connections1.nAChRs are pentameric complexes made up of combinations of a number of different nAChR subunits, which can be classified as α subunits and non-α subunits ('structural' subunits), which can be defined as β subunits when they are expressed in the vertebrate nervous system2. There are nine α subunits (α2-α10) and three β subunits (β2, β3, and β4) in the CNS3. Nicotinic receptors are assembled as combinations of α (2-6) and β (2-4) subunits. All α subunits are expressed in neuronal cells except for the α1 subunit which is specifically expressed in skeletal muscle. They are also expressed in non-neuronal cells such as bronchial epithelial cells4, as well lymphocytes5. In the CNS, the α5 subunit is widely expressed in the CA1 area of the hippocampus which affects learning, memory, attention, anxiety, and potentially drug seeking behaviors.Nicotinic acetylcholine receptor α5 is associated with different aspects of smoking behavior as well as with smoking related disorders. In addition, it has been found to be upregulated in smokers or differentially expressed in lung tumor cells6.

Applications may also work in :

Supplier :

Alomone Labs

Target :

nAChR α5, Cholinergic receptor nicotinic alpha 5, NACHRA5, Neuronal acetylcholine receptor subunit alpha-5

Short Description :

A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α5

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat nAChRα5. Anti-Nicotinic Acetylcholine Receptor α5 (CHRNA5) (extracellular) Antibody (#ANC-005) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize nAChRα5 from rat, mouse, and human samples.

Negative Control :

BLP-NC005

Positive Control :

Synonyms :

nAChR α5, Cholinergic receptor nicotinic alpha 5, NACHRA5, Neuronal acetylcholine receptor subunit alpha-5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CHRNA5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage