Anti-Nicotinic Acetylcholine Receptor α3 (CHRNA3) (extracellular) Antibody

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Nicotinic Acetylcholine Receptor α3 (CHRNA3) (extracellular) Antibody

catalog :

ANC-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-Nicotinic Acetylcholine Receptor α3 (CHRNA3) (extracellular) Antibody (#ANC-003), (1:200).2. Anti-Nicotinic Acetylcholine Receptor α3 (CHRNA3) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α3/CHRNA3 (extracellular) Blocking Peptide (#BLP-NC003).

image 2 :

Expression of Nicotinic acetylcholine receptor α3 in rat neocortex - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections were stained using Anti-Nicotinic Acetylcholine Receptor α3 (CHRNA3) (extracellular) Antibody (#ANC-003), (1:200). A. Intense staining (green) of nAChR α3 appears in apical dendrites (thin arrows). A few cortical interneurons (thick arrow) are also stained with the antibody. B. The same section was stained also for parvalbumin (red staining). An arrow depicts a neuron expressing both nAChR α3 and parvalbumin.

image 3 :

Expression of Nicotinic acetylcholine receptor ?3 in rat neocortex - Immunohistochemical staining of immersion-fixed free floating rat brain frozen sections were stained usingAnti-Nicotinic Acetylcholine Receptor ?3 (CHRNA3) (extracellular) Antibody(#ANC-003) (1:200). A. Intense staining (green) of nAChR ?3 appears in apical dendrites (thin arrows). A few cortical interneurons (thick arrow) are also stained with the antibody. B. The same section was stained also for parvalbumin (red staining). An arrow depicts a neuron expressing both nAChR ?3 and parvalbumin.

product information

CAT :

ANC-003

SKU :

ANC-003-CF_0.2 ml

Product Name :

Anti-Nicotinic Acetylcholine Receptor α3 (CHRNA3) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P04757

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NC003

Homology :

Mouse - identical; human - 11/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KWKPSDYQGVEFMR, corresponding to amino acid residues 91-104 of rat nAChRα3

Product Page - Scientific background :

Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs) which belong to the superfamily of G-protein coupled receptors (GPCRs) and nicotinic AChRs (nAChRs) which belong to the ligand-gated ion channel superfamily. nAChRs also respond to nicotine, hence their name1.To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of a subunits (α1-10), which is responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding2. The other subunits responsible for making up the active receptor are the β (β1-4), γ, δ and ε subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, 3 conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer of these various subunits organized around a central pore1.All α subunits are expressed in neuronal cells except for the α1 subunit which is specifically expressed in the muscle3. In the peripheral autonomic nervous system, nicotinic receptors are prominent and are responsible mediating fast synaptic transmission in all peripheral autonomic ganglia4. In this system, these channel receptors are mostly made up of two α3 subunits heteromerized with three other subunits. The α3 subunit plays a most important role in this system as knockout mice suffer from severe autonomic failure accompanied by gastrointestinal and bladder malfunctions5.Autoimmune autonomic ganglionopathy (AAG) is a neurological disorder characterized by dysfunctions in sympathetic, parasympathetic and enteric functions. Patients suffering from the disease express high levels of self antibodies against ionotropic nicotinic receptors, namely α3 subunits4. The α3 subunit is also found in dorsal root ganglion where it is most probably involved in nociception6.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

nAChR α3, Neuronal acetylcholine receptor subunit alpha-3, ACHA3, PAOD2

Short Description :

A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α3

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the extracellular N-terminal domain of the rat nAChRα3. Anti-Nicotinic Acetylcholine Receptor α3 (CHRNA3) (extracellular) Antibody (#ANC-003) can be used in western blot and immunohistochemistry applications. It has been designed to recognize nAChRα3 from mouse, rat and human samples.

Negative Control :

BLP-NC003

Positive Control :

Synonyms :

nAChR α3, Neuronal acetylcholine receptor subunit alpha-3, ACHA3, PAOD2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CHRNA3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage