Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody

catalog :

ANC-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Wang Z, Jung J, Inbar T, Rangoussis K, Faaborg Andersen C, Coate T. The Purinergic Receptor P2rx3 is Required for Spiral Ganglion Neuron Branch Refinement during Development. Eneuro. 2020;7: pubmed publisher
Takahashi T, Shiraishi A, Murata J. The Coordinated Activities of nAChR and Wnt Signaling Regulate Intestinal Stem Cell Function in Mice. Int J Mol Sci. 2018;19: pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: - 1,2. Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:400).3,4. Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α2/CHRNA2 (extracellular) Blocking Peptide (#BLP-NC002).

image 2 :

Expression of nAChRα2 in rat deep cerebellar nucleus - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:100). Staining reveals expression of nAChRα2 (red) in cells with neuronal outline (arrows point at some examples) in the red nucleus. DAPI is used as the counterstain (blue).

image 3 :

Peptide (C)DLEQMERTVDLKD corresponding to amino acid residues 189-201 of rat nAChR?2 (AccessionP12389). Extracellular N-terminus.

product information

CAT :

ANC-002

SKU :

ANC-002-CF_0.2 ml

Product Name :

Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P12389

Applications :

IC IF IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NC002

Homology :

Mouse - identical; human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DLEQMERTVDLKD, corresponding to amino acid residues 189-201 of rat nAChRα2

Product Page - Scientific background :

Nicotinic acetylcholine receptors (nAChRs) mediate the physiological effects of exogenous nicotine. They also play critical physiological roles throughout the brain and body by mediating cholinergic excitatory neurotransmission, modulating the release of neurotransmitters, and have longer-term effects on gene expression and cellular connections1.nAChRs are pentameric complexes made up of combinations of a number of different nAChR subunits, which can be classified as α subunits, containing two cysteine residues at positions analogous to Cys192 and Cys193, and non-alpha subunits ('structural' subunits), which can be defined as β subunits when they are expressed in the vertebrate nervous system2. There are nine α subunits (α2-α10) and three β subunits (β2, β3, and β4) in the CNS3. Nicotinic receptors are assembled as combinations of α (2-6) and and β (2-4) subunits. All α subunits are expressed in neuronal cells except for the α1 subunit which is specifically expressed in skeletal muscle. They are also expressed in non-neuronal cells such as bronchial epithelial cells4, as well lymphocytes5.In humans, a mutant α2 subunit has been identified, which forms nAChRs with increased agonist sensitivity and causes a form of familial epilepsy6. Further, an α2 subunit null mouse model has been used to demonstrate a role for α2 nAChR in nicotine-induced modulation of long-term potentiation in the mouse hippocampal CA1 region, which may underlie some of the cognitive effects of nicotine7.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

nAChR α2, Cholinergic receptor nicotinic alpha 2, Neuronal acetylcholine receptor subunit alpha-2

Short Description :

A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α2

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat nAChRα2. Anti-Nicotinic Acetylcholine Receptor α2 (extracellular) (CHRNA2) Antibody (#ANC-002) can be used in western blot, immunohistochemistry and immunocytochemistry applications. The antibody is specially suited to recognize nAChRα2 in live cells. It has been designed to recognize nAChRα2 from rat mouse and human samples.

Negative Control :

BLP-NC002

Positive Control :

Synonyms :

nAChR α2, Cholinergic receptor nicotinic alpha 2, Neuronal acetylcholine receptor subunit alpha-2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CHRNA2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage