Anti-Nicotinic Acetylcholine Receptor α1 (CHRNA1) (extracellular) Antibody

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Nicotinic Acetylcholine Receptor α1 (CHRNA1) (extracellular) Antibody

catalog :

ANC-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Kim C, Choi J, Joo S, Bae E, Ma S, Lee J, et al. Nicotine-Induced Apoptosis in Human Renal Proximal Tubular Epithelial Cells. PLoS ONE. 2016;11:e0152591 pubmed publisher

image

image 1 :

Western blot analysis of rat skeletal muscle (lanes 1 and 3) and mouse C2C12 myoblasts (lanes 2 and 4): - 1,2. Anti-Nicotinic Acetylcholine Receptor α1 (CHRNA1) (extracellular) Antibody (#ANC-001), (1:200).3,4. Anti-Nicotinic Acetylcholine Receptor α1 (CHRNA1) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α1/CHRNA1 (extracellular) Blocking Peptide (#BLP-NC001).

image 2 :

Peptide EHETRLVAKLFKD(C) corresponding to amino acid residues 22-34 of rat nAChR?1 (AccessionP25108). Extracellular N-terminus.

image 3 :

Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate: - 1. Anti-SLC17A9 Antibody (#ANT-085) (1:400).2. Anti-SLC17A9 Antibody preincubated with the negative control antigen.

product information

CAT :

ANC-001

SKU :

ANC-001-CF_0.2 ml

Product Name :

Anti-Nicotinic Acetylcholine Receptor α1 (CHRNA1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P25108

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NC001

Homology :

Human - identical; mouse - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

EHETRLVAKLFKD(C), corresponding to amino acid residues 22-34 of rat nAChRα1

Product Page - Scientific background :

Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs) which belong to the superfamily of G-protein coupled receptors (GPCRs), and nicotinic AChRs (nAChRs) which belong to the ligand-gated ion channel superfamily. nAChRs also respond to nicotine, hence their name1.To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of α subunits (α1-10), which is responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding2. The other subunits responsible for making up the active receptor are the β (β1-4), γ, δ and ε subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, 3 conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer (homopentamers also exist) of these various subunits organized around a central pore1.All α subunits are expressed in neuronal cells except for the α1 subunit which is specifically expressed in skeletal muscle. Indeed, two different nAChR structural entities with the following stoichiometry are observed in this tissue: (α1)2β1δγ in fetal muscle cells and (α1)2β1δε at mature neuromuscular synapses3.The α1 extracellular domain contains the main immunogenic region, a region against which a large fraction of autoantibodies against nAChR is directed in the autoimmune disease myasthenia gravis (MG)4. This autoimmune disease is characterized by the malfunction of neuromuscular transmission as a result of nonfunctional nAChRs, leading to defective signaling at the neuromuscular junction5.Due to its central role in muscle contraction and autonomic nervous system, nAChRs have evolved to be important targets of toxins secreted by plants, insects and animals. One prominent toxin affecting α1, in addition to other subunits, is α-bungarotoxin, a snake toxin which blocks nAChRs6.

Applications may also work in :

Supplier :

Alomone Labs

Target :

nAChR α1, ACHRA, Cholinergic receptor nicotinic alpha 1, SCCMS

Short Description :

A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α1

Long Description :

Anti-Nicotinic Acetylcholine Receptor α1 (CHRNA1) (extracellular) Antibody (#ANC-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize nAChRα1 from mouse, rat, and human samples.

Negative Control :

BLP-NC001

Positive Control :

Synonyms :

nAChR α1, ACHRA, Cholinergic receptor nicotinic alpha 1, SCCMS

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CHRNA1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage