Anti-NGF Antibody | Alomone Labs AN-240 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NGF Antibody

catalog :

AN-240

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 12

Reference
Rogdakis T, Charou D, Latorrata A, Papadimitriou E, Tsengenes A, Athanasiou C, et al. Development and Biological Characterization of a Novel Selective TrkA Agonist with Neuroprotective Properties against Amyloid Toxicity. Biomedicines. 2022;10: pubmed publisher
Belinskaia M, Zurawski T, Kaza S, Antoniazzi C, Dolly J, Lawrence G. NGF Enhances CGRP Release Evoked by Capsaicin from Rat Trigeminal Neurons: Differential Inhibition by SNAP-25-Cleaving Proteases. Int J Mol Sci. 2022;23: pubmed publisher
Zhang Y, Yin N, Sun A, Wu Q, Hu W, Hou X, et al. Transient Receptor Potential Channel 6 Knockout Ameliorates Kidney Fibrosis by Inhibition of Epithelial-Mesenchymal Transition. Front Cell Dev Biol. 2020;8:602703 pubmed publisher
SAS A, Carbajal K, Jerome A, Menon R, Yoon C, Kalinski A, et al. A new neutrophil subset promotes CNS neuron survival and axon regeneration. Nat Immunol. 2020;21:1496-1505 pubmed publisher
Elsayed H, Faroni A, Ashraf M, Osuji J, Wunderley L, Zhang L, et al. Development and Characterisation of an in vitro Model of Wallerian Degeneration. Front Bioeng Biotechnol. 2020;8:784 pubmed publisher
Danelon V, Goldner R, Martinez E, Gokhman I, Wang K, Yaron A, et al. Modular and Distinct Plexin-A4/FARP2/Rac1 Signaling Controls Dendrite Morphogenesis. J Neurosci. 2020;40:5413-5430 pubmed publisher
Pham D, Bruelle C, Thi Do H, Pajanoja C, Jin C, Srinivasan V, et al. Caspase-2 and p75 neurotrophin receptor (p75NTR) are involved in the regulation of SREBP and lipid genes in hepatocyte cells. Cell Death Dis. 2019;10:537 pubmed publisher
Shacham Silverberg V, Sar Shalom H, Goldner R, Golan Vaishenker Y, Gurwicz N, Gokhman I, et al. Phosphatidylserine is a marker for axonal debris engulfment but its exposure can be decoupled from degeneration. Cell Death Dis. 2018;9:1116 pubmed publisher
Elshaer S, El Remessy A. Deletion of p75NTR prevents vaso-obliteration and retinal neovascularization via activation of Trk- A receptor in ischemic retinopathy model. Sci Rep. 2018;8:12490 pubmed publisher
Unsain N, Bordenave M, Martínez G, Jalil S, von Bilderling C, Barabas F, et al. Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration. Sci Rep. 2018;8:3007 pubmed publisher
Kim M, Vargas M, Harlan B, Killoy K, Ball L, Comte Walters S, et al. Nitration and Glycation Turn Mature NGF into a Toxic Factor for Motor Neurons: A Role for p75NTR and RAGE Signaling in ALS. Antioxid Redox Signal. 2018;28:1587-1602 pubmed publisher
Mesentier Louro L, De Nicoló S, Rosso P, De Vitis L, Castoldi V, Leocani L, et al. Time-Dependent Nerve Growth Factor Signaling Changes in the Rat Retina During Optic Nerve Crush-Induced Degeneration of Retinal Ganglion Cells. Int J Mol Sci. 2017;18: pubmed publisher

image

image 1 :

Western blot analysis using Anti-NGF Antibody (#AN-240) (1:200).

image 2 :

Expression of NGF in rat brain - Immunohistochemical staining of rat hippocampal mossy fiber terminal zone using Anti-NGF Antibody (#AN-240). A. The mossy fiber terminal zone (MF) is seen as the gray area (arrow) adjacent to the pyramidal layer (P). The pyramidal layer is visualized using cresyl violet (purple-labeled cells). B. The antibody was pre-incubated with the immunogen. The lack of staining in the MF zone (arrow) demonstrates specificity.

image 3 :

Expression of NGF in rat brain - Immunohistochemical staining of rat hippocampal mossy fiber terminal zone usingAnti-NGFAntibody (#AN-240). A. The mossy fiber terminal zone (MF) is seen as the gray area (arrow) adjacent to the pyramidal layer (P). The pyramidal layer is visualized using cresyl violet (purple-labeled cells). B. The antibody was pre-incubated with the immunogen. The lack of staining in the MF zone (arrow) demonstrates specificity.

product information

CAT :

AN-240

SKU :

AN-240-CF_0.2 ml

Product Name :

Anti-NGF Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P01139

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-N240

Homology :

Rat- 115/120 amino acid residues identical; human- 107/120 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by DNA sequence and SDS-PAGE

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

Highly purified 2.5 S mouse NGF

Product Page - Scientific background :

The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and the PNS.1 NGF, the first member of the family to be discovered, was originally purified as a factor able to support survival of sympathetic and sensory spinal neurons in culture.2 It is synthesized and secreted by sympathetic and sensory target organs and provides trophic support to neurons as they reach their final target.3 Neurotrophin secretion also increases in the nervous system following injury. Schwann cells, fibroblasts, and activated mast cells normally synthesize NGF constitutively, however direct trauma and induced cytokines combine to increase neurotrophin production in these cells after injury.4NGF is purified in three forms: the 7S, 2.5S and β. The 7S, 130 kDa, form occurs naturally in mouse submaxillary glands, and is a multimeric protein composed of two α, one β and two γ subunits. The name is derived from its sedimentation co-efficient, 7S. The biologically active subunit is the β, which is a 26 kDa dimer composed of two identical 120 amino acid chains held together by hydrophobic interactions.5 The 2.5S form is 9 amino acids shorter than the β form, because of proteolysis that occurs during the purification process.6 The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF.7 There is a 95.8% homology between the rat and mouse forms, and a 85% homology between the human and mouse.NGF has been shown to regulate neuronal survival, development function and plasticity.8 Recently, involvement of NGF in processes not involving neuronal cells has been shown, such as asthma,9 psoriasis10 and wound healing.11The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75, which binds all the neurotrophins.12

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Nerve growth factor

Short Description :

A Rabbit Polyclonal Antibody to Nerve Growth Factor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against mouse NGF. Anti-NGF Antibody (#AN-240) can be used in western blot and immunohistochemical applications. It has been designed to recognize NGF from mouse, rat and human samples. The antibody also recognizes proNGF. Related products for neutralizing NGF activity: Mouse NGF/proNGF Neutralizing Antibody (#ALM-006) can be used in indirect ELISA and neutralization assays to block the biological effects of both NGF and proNGF.

Negative Control :

BLP-N240

Positive Control :

Synonyms :

Nerve growth factor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NGF

AB Specifictiy :

The antibody does not react with BDNF, NT-3, and NT-4/5.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage