Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody | Alomone Labs AN-170 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody

catalog :

AN-170

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

192-IgG.

reactivity :

rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; rat; 1:2000; loading ...; fig 1f	Koike T, Tanaka S, Hirahara Y, Oe S, Kurokawa K, Maeda M, et al. Morphological characteristics of p75 neurotrophin receptor-positive cells define a new type of glial cell in the rat dorsal root ganglia. J Comp Neurol. 2019;527:2047-2060 pubmed publisher
	Salvo E, Tu N, Scheff N, Dubeykovskaya Z, Chavan S, Aouizerat B, et al. TNFα promotes oral cancer growth, pain, and Schwann cell activation. Sci Rep. 2021;11:1840 pubmed publisher
	Olianas M, Dedoni S, Onali P. Antidepressants induce profibrotic responses via the lysophosphatidic acid receptor LPA1. Eur J Pharmacol. 2020;873:172963 pubmed publisher
	El Habta R, Kingham P, Backman L. Adipose stem cells enhance myoblast proliferation via acetylcholine and extracellular signal-regulated kinase 1/2 signaling. Muscle Nerve. 2018;57:305-311 pubmed publisher
	Chen W, Sheng J, Guo J, Gao F, Zhao X, Dai J, et al. Tumor necrosis factor-Î± enhances voltage-gated Naâº currents in primary culture of mouse cortical neurons. J Neuroinflammation. 2015;12:126 pubmed publisher
	Mitrofanova L, Gorshkov A, Lebedev D, Mikhaylov E. Evidence of specialized tissue in human interatrial septum: histological, immunohistochemical and ultrastructural findings. PLoS ONE. 2014;9:e113343 pubmed publisher
	Hsu H, Tang N, Lin Y, Li T, Liu H, Hsieh C. Effect of electroacupuncture on rats with chronic constriction injury-induced neuropathic pain. ScientificWorldJournal. 2014;2014:129875 pubmed publisher
	Tan J, Shepherd R. Aminoglycoside-induced degeneration of adult spiral ganglion neurons involves differential modulation of tyrosine kinase B and p75 neurotrophin receptor signaling. Am J Pathol. 2006;169:528-43 pubmed
	Kuwako K, Hosokawa A, Nishimura I, Uetsuki T, Yamada M, Nada S, et al. Disruption of the paternal necdin gene diminishes TrkA signaling for sensory neuron survival. J Neurosci. 2005;25:7090-9 pubmed
	Tcherpakov M, Bronfman F, Conticello S, Vaskovsky A, Levy Z, Niinobe M, et al. The p75 neurotrophin receptor interacts with multiple MAGE proteins. J Biol Chem. 2002;277:49101-4 pubmed

image

image 1 :

Western blot analysis of PC12 cell lysate (non-reduced): - 1. Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody (#AN-170), (1:2500).2. Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody (1:1500).3. Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody (1:150).

image 2 :

Multiplex staining of Plexin-A2 and p75NTR in rat brain - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Plexin-A2 (extracellular) Antibody (#APR-082), (1:400) and Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody (#AN-170), (1:300). A. Sections of rat horizontal diagonal band (HDB) were stained for Plexin-A2 (green). B. Same sections were stained for p75NTR (red). C. Merge of the two images reveals cells expressing both Plexin-A2 and p75NTR (horizontal arrows), cells expressing only Plexin-A2 (upwards pointing arrows) or cells expressing only p75NTR (downwards pointing arrows) can also be observed. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AN-170

SKU :

AN-170_10 mcg

Product Name :

Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Monoclonal

Accession :

Q08201

Applications :

IF IFC IHC WB

Reactivity :

Rat

Host :

Mouse

Blocking Peptide :

Homology :

Recognizes p75NTR from rat samples only

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

IgG1

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

10 μl, 50 μl, 100 μl or 250 μl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

Solubilized proteins from PC12 membranes.

Product Page - Scientific background :

p75NTR known as a cell surface receptor for NGF, BDNF, NT-3 and NT-4, is also a receptor for b-amyloid, and for the myelin axon repellant protein MAG.1,2,4,6 Binding of neurotrophins induces receptor dimerization followed by phosphorylation of receptor kinase residues and recruitment of intracellular proteins involved in signal transduction. p75NTR functions alone, or in functional complexes with the neurotrophin receptors trkA, trkB and trkC. p75NTR signals via interaction with different transducers such as TRAF6, IRAK, and RIP-2, activating NF-κB, JNK and p38, and inactivating rhoA.1,3,6 Receptor degradation is mediated by a metalloproteinase-dependent shedding of the extracellular domain. p75NTR binds all neurotrophins with similar nM affinity, but binds pro-neurotrophins with greater affinity, and is more potently activated by pro-neurotrophins.5Overexpression of the intracellular domain in developing neurons induces apoptosis. NGF-dependent association of NRIF with p75NTR contributes to pro-apoptotic signaling.7,8 Beta-amyloid peptide binds p75NTR and stimulates pro apoptotic signaling by p75NTR, suggesting that the receptor may be relevant for Alzheimer's disease.9

Applications may also work in :

IF IFC IHC WB

Supplier :

Alomone Labs

Target :

p75NTR, NGFR p75, TNFR superfamily member 16, TNFRSF16, CD271

Short Description :

A Mouse Monoclonal Antibody to Rat Neurotrophin Receptor p75NTR

Long Description :

Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody (#AN-170) is a highly specific monoclonal antibody directed against p75NTR. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize p75NTR from rat samples only.

Negative Control :

Positive Control :

Synonyms :

p75NTR, NGFR p75, TNFR superfamily member 16, TNFRSF16, CD271

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NGFR

AB Specifictiy :

192-IgG reacts with rat p75NTR. It does not cross-react with p75NTR of human or mouse origin.

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

192-IgG.

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:150-1:2500

more info or order :

Alomone Labs product webpage