Mouse Anti-Rat p75 NGF Receptor (extracellular)-FITC Antibody | Alomone Labs AN-170-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Mouse Anti-Rat p75 NGF Receptor (extracellular)-FITC Antibody

catalog :

AN-170-F

clonality :

monoclonal

host :

mouse

conjugate :

FITC

clone name :

192-IgG.

reactivity :

rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Salvo E, Tu N, Scheff N, Dubeykovskaya Z, Chavan S, Aouizerat B, et al. TNFα promotes oral cancer growth, pain, and Schwann cell activation. Sci Rep. 2021;11:1840 pubmed publisher
Tezza S, Ben Nasr M, D Addio F, Vergani A, Usuelli V, Falzoni S, et al. Islet-Derived eATP Fuels Autoreactive CD8+ T Cells and Facilitates the Onset of Type 1 Diabetes. Diabetes. 2018;67:2038-2053 pubmed publisher
D Addio F, Vergani A, Potena L, Maestroni A, Usuelli V, Ben Nasr M, et al. P2X7R mutation disrupts the NLRP3-mediated Th program and predicts poor cardiac allograft outcomes. J Clin Invest. 2018;128:3490-3503 pubmed publisher
Castillo Azofeifa D, Losacco J, Salcedo E, Golden E, Finger T, Barlow L. Sonic hedgehog from both nerves and epithelium is a key trophic factor for taste bud maintenance. Development. 2017;144:3054-3065 pubmed publisher

image

image 1 :

Cell surface detection of p75NTR in live PC12 cells: Cell surface detection of p75NTR in live PC12 cells: Black line: Unstained cells. Green line: Cells + Mouse Anti-Rat p75 NGF Receptor (extracellular)-FITC Antibody (#AN-170-F) 1 µl/1x106 cells (1:50).

image 2 :

Cell surface detection of p75NTR in livePC12 cells: - ___Unstained cells.___Cells + Mouse Anti-Rat p75NGF Receptor (extracellular)-FITC Antibody(#AN-170-F) 1 l/1x106cells (1:50).

product information

CAT :

AN-170-F

SKU :

AN-170-F_50 mcg

Product Name :

Mouse Anti-Rat p75 NGF Receptor (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Monoclonal

Accession :

Q08201

Applications :

Reactivity :

Rat

Host :

Mouse

Blocking Peptide :

Homology :

Recognizes p75NTR from rat samples only

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

IgG1

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

Solubilized proteins from PC12 membranes.

Product Page - Scientific background :

p75NTR known as a cell surface receptor for NGF, BDNF, NT-3 and NT-4, is also a receptor for β-amyloid, and for the myelin axon repellant protein MAG.1,2,4,6 Binding of neurotrophins induces receptor dimerization followed by phosphorylation of receptor kinase residues and recruitment of intracellular proteins involved in signal transduction. p75NTR functions alone, or in functional complexes with the neurotrophin receptors trkA, trkB and trkC. p75NTR signals via interaction with different transducers such as TRAF6, IRAK, and RIP-2, activating NF-κB, JNK and p38, and inactivating rhoA.1,3,6 Receptor degradation is mediated by a metalloproteinase-dependent shedding of the extracellular domain. p75NTR binds all neurotrophins with similar nM affinity, but binds pro-neurotrophins with greater affinity, and is more potently activated by pro-neurotrophins.5 Overexpression of the intracellular domain in developing neurons induces apoptosis. NGF-dependent association of NRIF with p75NTR contributes to pro-apoptotic signaling.7,8 β-amyloid peptide binds p75NTR and stimulates pro-apoptotic signaling by p75NTR, suggesting that the receptor may be relevant for Alzheimer's disease.9

Applications may also work in :

Supplier :

Alomone Labs

Target :

p75NTR, NGFR p75, TNFR superfamily member 16, TNFRSF16, CD271

Short Description :

A Mouse Monoclonal Antibody to Rat Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye FITC

Long Description :

Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody (#AN-170) is a highly specific monoclonal antibody directed against p75NTR. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize p75NTR from rat samples only. Mouse Anti-Rat p75 NGF Receptor (extracellular)-FITC Antibody (#AN-170-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody was specifically developed to be used in direct flow cytometry applications, especially for those that use multiple and simultaneous labeling of different markers.

Negative Control :

Positive Control :

Synonyms :

p75NTR, NGFR p75, TNFR superfamily member 16, TNFRSF16, CD271

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NGFR

AB Specifictiy :

192-IgG reacts with rat p75NTR. It does not cross-react with p75NTR of human or mouse origin.

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

192-IgG.

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AN-170), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage