Anti-ABCB4/MDR3 Antibody | Alomone Labs AMT-023 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ABCB4/MDR3 Antibody

catalog :

AMT-023

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse liver lysate (lanes 1 and 3) and rat liver membranes (lanes 2 and 4): - 1,2. Anti-ABCB4/MDR3 Antibody (#AMT-023), (1:200).3,4. Anti-ABCB4/MDR3 Antibody, preincubated with ABCB4/MDR3 Blocking Peptide (#BLP-MT023).

product information

CAT :

AMT-023

SKU :

AMT-023-CF_0.2 ml

Product Name :

Anti-ABCB4/MDR3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q08201

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MT023

Homology :

Mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KARIFRNSTKKSLK, corresponding to amino acid residues 658-671 of rat ABCB4/MDR3

Product Page - Scientific background :

ABCB4 (MDR3) is a member of the ABC transporter superfamily. ABC transporters comprise a large number of membrane proteins, which mediate the translocation of a variety of molecules across cellular membranes. MDR3 is a transporter expressed at the canalicular membrane of hepatocytes, where it translocates phosphatidylcholine (PC) in bile in order to protect the hepatobiliary epithelial against the damage from free bile acids1-3.Genetic variants in the ABCB4 gene sequence are associated with rare biliary diseases, in particular progressive familial intrahepatic cholestasis type 3. Different phenotypes are detected in ABCB4 mutations such as, low phospholipid-associated cholelithiasis and intrahepatic cholestasis of pregnancy1-3.ABCB4 is a polytopic transmembrane glycoprotein with two transmembrane modules each spanning the membrane six times and two ATP-binding domains. The protein possesses a conserved C-terminal region. The presence of a hydrophobic amino acid at the C-terminus suggests properties of a PDZ-binding-like motif2,3.

Applications may also work in :

Supplier :

Alomone Labs

Target :

ATP binding cassette subfamily B member 4, Multidrug resistance protein 3, P-glycoprotein 2, Pgy2

Short Description :

A Rabbit Polyclonal Antibody to ABCB4/MDR3

Long Description :

Anti-ABCB4/MDR3 Antibody (#AMT-023) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize MDR3 from rat and mouse samples. It is unlikely to recognize the protein from human samples.

Negative Control :

BLP-MT023

Positive Control :

Synonyms :

ATP binding cassette subfamily B member 4, Multidrug resistance protein 3, P-glycoprotein 2, Pgy2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ABCB4

AB Specifictiy :

Not recommended for human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage