Anti-Human ABCB1/MDR1 Antibody | Alomone Labs AMT-021 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human ABCB1/MDR1 Antibody

catalog :

AMT-021

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Chelakkot V, Liu K, Yoshioka E, Saha S, Xu D, Licursi M, et al. MEK reduces cancer-specific PpIX accumulation through the RSK-ABCB1 and HIF-1α-FECH axes. Sci Rep. 2020;10:22124 pubmed publisher
Beaino W, Janssen B, Kooij G, van der Pol S, Van Het Hof B, van Horssen J, et al. Purinergic receptors P2Y12R and P2X7R: potential targets for PET imaging of microglia phenotypes in multiple sclerosis. J Neuroinflammation. 2017;14:259 pubmed publisher

image

image 1 :

Western blot analysis of human LNCaP prostate carcinoma (lanes 1 and 4), human HeLa cervix carcinoma (lanes 2 and 5) and human HepG2 liver carcinoma (lanes 3 and 6) cell line lysates: - 1-3. Anti-Human ABCB1/MDR1 Antibody (#AMT-021), (1:200).4-6. Anti-Human ABCB1/MDR1 Antibody, preincubated with Human ABCB1/MDR1 Blocking Peptide (#BLP-MT021).

product information

CAT :

AMT-021

SKU :

AMT-021-CF_0.2 ml

Product Name :

Anti-Human ABCB1/MDR1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P08183

Applications :

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-MT021

Homology :

Not recommended for rat or mouse samples

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)RGSQAQDRKLSTKE, corresponding to amino acid residues 673-686 of human multidrug resistance protein 1

Product Page - Scientific background :

Multidrug resistance protein 1 (MRP1, also known as P glycoprotein 1) is a member of a superfamily of proteins known as the ATP-binding cassette (ABC) transporters. This superfamiy is classified into seven subfamilies (A through G). Subfamily ABCC is composed of 12 proteins and includes MRP1 and is encoded by ABCC1.MRP1 structure comprises five domains with two nucleotide binding domains (NBDs) that bind ATP, and 12 transmembrane domains.MRP1 mediates the ATP-dependent efflux of a variety of solutes across membranes against a concentration gradient. These solutes include antineoplastic agents such as vincristine and doxorubicin, physiological organic anions such as the antioxidant GSH and the pro-inflammatory cysteinyl leukotriene C4, hydrophilic conjugated xenobiotic, various antibiotics, opiates, antiviral agents, citalopram, and statins. MRP1 is the only MRP-related protein with an acknowledged role in tumor multidrug resistance in clinical oncology. It also plays an important role in the efficacy and toxicity of drugs used for the treatment of nonmalignant diseasese1,2.Knock-out mouse model of Abcc1−/− has shown that MRP1 can act as an important factor of drug disposition because of its presence in cells at the interface between many tissues. The protein also demonstrates chemoprotective properties1.Evidence suggests that ABC transporters also have an important role in normal cellular biology including regulation of cell differentiation, proliferation, and survival, including apoptosis, tumor promotion and modulation of immune function2.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Multidrug resistance protein 1, ATP-binding cassette sub-family B (MDR/TAP) member 1, P-glycoprotein 1, P-gp, PGY1, ABC20, CD243, CLCS, Colchicin sensitivity, Doxorubicin resistance, GP170

Short Description :

A Rabbit Polyclonal Antibody to Multidrug Resistance Protein 1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human multidrug resistance protein 1. Anti-Human ABCB1/MDR1 Antibody (#AMT-021) can be used in western blot analysis. It has been designed to recognize MDR1 from human samples only. The antibody is specific for MDR1 and will not cross-react with the highly homologous MDR3 protein.

Negative Control :

BLP-MT021

Positive Control :

Synonyms :

Multidrug resistance protein 1, ATP-binding cassette sub-family B (MDR/TAP) member 1, P-glycoprotein 1, P-gp, PGY1, ABC20, CD243, CLCS, Colchicin sensitivity, Doxorubicin resistance, GP170

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ABCB1

AB Specifictiy :

Not recommended for rat or mouse samples. The antibody is specific for MDR1. It won’t cross-react with the highly homologous MDR3 protein.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage