Anti-MCT4 (SLC16A3) Antibody | Alomone Labs AMT-014 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-MCT4 (SLC16A3) Antibody

catalog :

AMT-014

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Silagi E, Novais E, Bisetto S, Telonis A, Snuggs J, Le Maitre C, et al. Lactate Efflux From Intervertebral Disc Cells Is Required for Maintenance of Spine Health. J Bone Miner Res. 2019;: pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-MCT4 (SLC16A3) Antibody (#AMT-014), (1:200).3,4. Anti-MCT4 (SLC16A3) Antibody, preincubated with MCT4/SLC16A3 Blocking Peptide (#BLP-MT014).

product information

CAT :

AMT-014

SKU :

AMT-014-CF_0.2 ml

Product Name :

Anti-MCT4 (SLC16A3) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O35910

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MT014

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)REVEHFLKAEPEK, corresponding to amino acid residues 447-459 of rat MCT4

Product Page - Scientific background :

Monocarboxylate transporter 4 (MCT4) belongs to a group of membrane proteins responsible for mediating the transfer of various short chain monocarboxylates including lactate, pyruvate, and ketones across the cell membrane. MCTs also transfer H+ resulting in acidification of the tissue microenvironment, thus playing an important role in the control of intracellular pH by proton-coupled release. MCT4 transports monocarboxylates in a ratio of 1:1 with H+.Transport activity of MCT4 is enhanced by carbonic anhydrase II, which has been suggested to function as a "proton antenna" for MCT41-3.The SLC16 gene family of MCTs comprises 14 members with a structure that contains 12 transmembrane domains. Among MCT members, only MCT1 and MCT4 catalyze the proton-coupled transport of lactate and H+. The distribution of MCTs is different by cell type. MCT4 is highly expressed in glycolytic cells, such as skeletal muscle fibers, astrocytes, leukocytes, chondrocytes, and some mammalian cell lineages.MCT4 is extensively investigated in cancer cells and is considered to be a metabolic target for anticancer therapies2-4.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Monocarboxylate transporter 4, Monocarboxylic acid transporter 4, MOT4

Short Description :

A Rabbit Polyclonal Antibody to Monocarboxylate Transporter 4

Long Description :

Anti-MCT4 (SLC16A3) Antibody (#AMT-014) is a highly specific antibody directed against an epitope of the rat Monocarboxylate transporter 4. The antibody can be used in western blot analysis. It has been designed to recognize MCT4 from human, rat, and mouse samples.

Negative Control :

BLP-MT014

Positive Control :

Synonyms :

Monocarboxylate transporter 4, Monocarboxylic acid transporter 4, MOT4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC16A3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage