Anti-MCT3 Antibody | Alomone Labs AMT-013 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-MCT3 Antibody

catalog :

AMT-013

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:200; loading ...; fig 2c western blot; mouse; 1:1000; loading ...; fig 2d	Shang P, Stepicheva N, Teel K, McCauley A, Fitting C, Hose S, et al. βA3/A1-crystallin regulates apical polarity and EGFR endocytosis in retinal pigmented epithelial cells. Commun Biol. 2021;4:850 pubmed publisher
	Pentz R, Iulita M, Ducatenzeiler A, Videla L, Benejam B, Carmona Iragui M, et al. Nerve growth factor (NGF) pathway biomarkers in Down syndrome prior to and after the onset of clinical Alzheimer's disease: A paired CSF and plasma study. Alzheimers Dement. 2021;17:605-617 pubmed publisher

image

image 1 :

Western blot analysis of rat whole eye lysate (lanes 1 and 3) and rat hippocampus (lanes 2 and 4): - 1,2. Anti-MCT3 Antibody (#AMT-013), (1:200).3,4. Anti-MCT3 Antibody, preincubated with MCT3 Blocking Peptide (#BLP-MT013).

product information

CAT :

AMT-013

SKU :

AMT-013-CF_0.2 ml

Product Name :

Anti-MCT3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O70461

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MT013

Homology :

Mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RSAEGGASDPEDVE, corresponding to amino acid residues 421-434 of rat MCT3

Product Page - Scientific background :

Proton-linked monocarboxylate transporters (MCTs) are a family of highly homologous trans-membrane symporters, involved in the transportation of monocarboxylic acids across the plasma membrane such as: lactate, pyruvate, branched-chain oxoacids derived from leucine, valine, and isoleucine, ketone bodies, acetoacetate, β-hydroxybutyrate and acetate. Thus, MCTs play an important role in metabolic communication among cells and in mammalian metabolism.MCT3 is encoded by the Slc16a8 gene and contains at least nine members in humans1. Structurally, MCTs have 12 transmembrane spanning domains with cytoplasmic NH2- and COOH-terminals. The COOH-terminal regions are not well conserved among the MCTs isoforms while the membrane spanning domains share the greatest sequence identity3,4. Findings suggest that the N-terminal domain is more important for energy coupling, membrane insertion and correct structure maintenance, whereas the C-terminal domain is more important for the determination of substrate specificity1.MCT3 is expressed in the retinal pigment epithelium, preferentially expressed in the basolateral membrane in the adult and in choroid plexus epithelium where it plays a role in regulating pH and lactate concentrations in the outer retina. Reduced levels of MCT3 in response to trauma or disease could contribute to pathologic changes in the retina2,3. MCT3 can form a heteromeric complex with the accessory protein CD147, an immunoglobulin superfamily protein.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Monocarboxylate transporter 3, Monocarboxylic acid transporter 3, SLC16A8

Short Description :

A Rabbit Polyclonal Antibody to Monocarboxylate Transporter 3

Long Description :

Anti-MCT3 Antibody (#AMT-013) is a highly specific antibody directed against an epitope of the rat Monocarboxylate transporter 3. The antibody can be used in western blot analysis. It has been designed to recognize MCT3 from rat and mouse samples. The antibody is unlikely to recognize the protein from human samples.

Negative Control :

BLP-MT013

Positive Control :

Synonyms :

Monocarboxylate transporter 3, Monocarboxylic acid transporter 3, SLC16A8

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC16A8

AB Specifictiy :

Not recommended for human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage