Anti-MCT2 Antibody | Alomone Labs AMT-012 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-MCT2 Antibody

catalog :

AMT-012

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse testis membranes: - 1. Anti-MCT2 Antibody (#AMT-012), (1:200).2. Anti-MCT2 Antibody, preincubated with MCT2 Blocking Peptide (#BLP-MT012).

image 2 :

Expression of MCT2 in mouse brain sections - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-MCT2 Antibody (#AMT-012), (1:1000). A. MCT2 staining in mouse cortex sections (red) appears in neuronal outlines (arrows). B. MCT2 staining in dorsal pons brain stem regions (red) appears in neuronal outlines (horizontal arrows) and in fibers (vertical arrows). 4th V = fourth ventricle. Cell nuclei are stained with DAPI (blue).

image 3 :

Peptide (C)KEMEALSRSKQDD corresponding to amino acid residues 456-468 of rat MCT2 (AccessionQ63344). Intracellular C-terminus.

product information

CAT :

AMT-012

SKU :

AMT-012-CF_0.2 ml

Product Name :

Anti-MCT2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63344

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MT012

Homology :

Mouse - 10/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KEMEALSRSKQDD, corresponding to amino acid residues 456-468 of rat MCT2

Product Page - Scientific background :

The SLC16A family of monocarboxylate transporters (MCTs) is composed of 14 members. MCTs play a central role in cellular metabolism and metabolic communication between tissues. The rapid transport of monocarboxylates such as pyruvate, lactate, and ketone bodies (acetoacetate and β-hydroxybutyrate) across the plasma membrane of cells is facilitated by proton-linked MCTs1. MCTs share similarities in sequence and protein structure, but each member differs from others in the substrates that they transport. For example, lactate among other monocarboxylates, is the primary substrate for MCT1, MCT2, MCT3 and MCT42. All family members are predicted to have 12 transmembrane helices (TMs) with intracellular C- and N-termini as well as a large cytosolic loop3.MCT2 is distributed in testis, brain, skeletal muscle, heart, and kidney4.MCTs are important for normal metabolic function and are implicated in disease. Changes in the cerebral density of MCT2 have been reported acutely following hypoxia-ischemia in vivo and in hippocampal slices5.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Monocarboxylate transporter 2, Monocarboxylic acid transporter 2, SLC16A7

Short Description :

A Rabbit Polyclonal Antibody to Monocarboxylate Transporter 2

Long Description :

Anti-MCT2 Antibody (#AMT-012) is a highly specific antibody directed against an epitope of the rat Monocarboxylate transporter 2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MCT2 from rat and mouse samples. The antibody is unlikely to recognize the protein from human samples.

Negative Control :

BLP-MT012

Positive Control :

Synonyms :

Monocarboxylate transporter 2, Monocarboxylic acid transporter 2, SLC16A7

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC16A7

AB Specifictiy :

Not recommended for human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1000

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage