antibody

Anti-MCT1 (SLC16A1) (extracellular) Antibody

AMT-011

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry, immunocytochemistry

AMT-011-CF_0.2 ml

Anti-MCT1 (SLC16A1) (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

P53987

IF IFC IHC LCI WB

Human Rat Mouse

Rabbit

BLP-MT011

Mouse, human - identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

6th extracellular loop

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)GRLNDMYGDYKYT, corresponding to amino acid residues 403-415 of rat MCT1

Monocarboxylates such as pyruvate, lactate, and ketone bodies play essential roles in carbohydrate, fat, and amino acid metabolism and must be rapidly transported across the plasma membrane of cells. Transport of monocarboxylates is mediated by proton-linked monocarboxylate transporters (MCTs).MCT1 is comprised of 12-transmembrane helices (TMs) with intracellular C- and N-termini and a large cytosolic loop between TMs 6 and 7. The TM regions are more conserved than the loops and C-terminus. MCT1 demonstrates Michaelis Menten kinetics with a broad specificity for short-chain monocarboxylates such as halides, hydroxyl, and carbonyl groups. In addition, the transport of unsubstituted short-chain fatty acids, such as acetate, propionate, and butyrate, is strongly facilitated by MCT1. Natural occurring substrates for MCT1 include L-lactate, pyruvate, β-hydroxybutyrate, and acetoacetate. The predominant role of MCT1 is to facilitate unidirectional proton-linked transport of L-lactate across the plasma membrane. This may result in either influx or efflux of lactic acid depending of the prevailing intracellular and extracellular substrate concentrations and the pH gradient across the plasma membrane. When transporting lactate into the cell, MCT1 has a substrate binding site open to the extracellular matrix which binds a proton first followed by the lactate anion1.MCT1 has been considered as a promising target for cancer therapy, since it facilitates lactate efflux in glycolytic tumors. In breast carcinoma, MCT1 is associated with poor prognostic variables such as basal-like subtype and high grade tumors2.

IF IFC IHC LCI WB

Alomone Labs

Monocarboxylate transporter 1, Monocarboxylic acid transporter 1

A Rabbit Polyclonal Antibody to Monocarboxylate Transporter 1

Anti-MCT1 (SLC16A1) (extracellular) Antibody (#AMT-011) is a highly specific antibody directed against an epitope of rat Monocarboxylate transporter 1. The antibody can be used in western blot, immunohistochemistry, and indirect live cell flow cytometry applications. It has been designed to recognize MCT1 from rat, mouse, and human samples.

BLP-MT011

NA

Monocarboxylate transporter 1, Monocarboxylic acid transporter 1

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

SLC16A1

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

yes

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:200

1:200