Anti-MCT1 (SLC16A1) (extracellular)-APC Antibody | Alomone Labs AMT-011-APC product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-MCT1 (SLC16A1) (extracellular)-APC Antibody

catalog :

AMT-011-APC

clonality :

polyclonal

host :

domestic rabbit

conjugate :

APC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of monocarboxylate transporter 1 by direct flow cytometry in live intact mouse J774 macrophage cells: Cell surface detection of monocarboxylate transporter 1 by direct flow cytometry in live intact mouse J774 macrophage cells: Black line: Cells. Red line: Cells + Rabbit IgG Isotype control-APC (#RIC-001-APC). Green line: Cells + Anti-MCT1 (SLC16A1) (extracellular)-APC Antibody (#AMT-011-APC), (1 µg).

product information

CAT :

AMT-011-APC

SKU :

AMT-011-APC_50 mcl

Product Name :

Anti-MCT1 (SLC16A1) (extracellular)-APC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P53987

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MT011

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

6th extracellular loop

Label :

Allophycocyanin (APC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)GRLNDMYGDYKYT, corresponding to amino acid residues 403-415 of rat MCT1

Product Page - Scientific background :

Monocarboxylates such as pyruvate, lactate, and ketone bodies play essential roles in carbohydrate, fat, and amino acid metabolism and must be rapidly transported across the plasma membrane of cells. Transport of monocarboxylates is mediated by proton-linked monocarboxylate transporters (MCTs).MCT1 is comprised of 12-transmembrane helices (TMs) with intracellular C- and N-termini and a large cytosolic loop between TMs 6 and 7. The TM regions are more conserved than the loops and C-terminus. MCT1 demonstrates Michaelis Menten kinetics with a broad specificity for short-chain monocarboxylates such as halides, hydroxyl, and carbonyl groups. In addition, the transport of unsubstituted short-chain fatty acids, such as acetate, propionate, and butyrate, is strongly facilitated by MCT1. Natural occurring substrates for MCT1 include L-lactate, pyruvate, β-hydroxybutyrate, and acetoacetate. The predominant role of MCT1 is to facilitate unidirectional proton-linked transport of L-lactate across the plasma membrane. This may result in either influx or efflux of lactic acid depending of the prevailing intracellular and extracellular substrate concentrations and the pH gradient across the plasma membrane. When transporting lactate into the cell, MCT1 has a substrate binding site open to the extracellular matrix which binds a proton first followed by the lactate anion1.MCT1 has been considered as a promising target for cancer therapy, since it facilitates lactate efflux in glycolytic tumors. In breast carcinoma, MCT1 is associated with poor prognostic variables such as basal-like subtype and high grade tumors2.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Monocarboxylate transporter 1, Monocarboxylic acid transporter 1

Short Description :

A Rabbit Polyclonal Antibody to MCT1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)

Long Description :

Anti-MCT1 (SLC16A1) (extracellular) Antibody (#AMT-011) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and indirect live cell flow cytometry. It has been designed to recognize MCT1 from rat, mouse and human samples. Anti-MCT1 (SLC16A1) (extracellular)-APC Antibody (#AMT-011-APC) is directly conjugated to Allophycocyanin (APC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Negative Control :

RIC-001-APC

Positive Control :

Synonyms :

Monocarboxylate transporter 1, Monocarboxylic acid transporter 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC16A1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AMT-011), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage